FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204257
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT22511.1020430144376936No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6300.3084349618372932No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.25458123834189283No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5080.24870628668784914No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT4830.23646680407525814No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.22226900424465257No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC4470.2188419491131271No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.18310265988436136No Hit
GTATCAACGCAGAGTACGGGAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTC3520.1723319151852813No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGA3520.1723319151852813No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.16547780492223033No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.15960285326818666No Hit
AAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGG3130.15323832230963932No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.1527487430051357No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.1503008464826175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.14981126717811385No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGG2780.13610304665201192No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.1307176743024719No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2660.13022809499796825No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2660.13022809499796825No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.12973851569346462No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2570.1258218812574355No Hit
CAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCC2520.12337398473491729No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.12288440543041365No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC2440.11945735029888817No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCG2420.11847819168988089No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.11700945377636997No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.11700945377636997No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.11651987447186633No Hit
GTATCAACGCAGAGTACGGGATGGTGACCACGGGTGACGGGGAATCAGGG2370.11603029516736268No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG2330.11407197794934812No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2230.10917618490431172No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA2060.10085333672774983No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGTT253.9041098E-4108.3707170
AGGGGGG3850.089.1360870
TCGGAGA350.001486628877.40764670
AAGGGGG3200.069.3052869
AATCCCG150.003543347862.22617730
TTGCATG150.004240304759.4690363
AAAGGGG3600.057.3816668
CGTCCTC250.004902039757.31757765
GCTTGAA4400.057.0887556
TAGATAG200.00522493956.40812744
TGCTTGA4450.055.8405555
CCGTCTT4100.055.54236647
TCTTCTG4100.054.6456250
CTTGAAA4700.053.99361457
AAAAAGG3750.053.0572466
AAAAGGG3900.052.07769467
GCCGTCT4200.051.7082946
CGTCTTC4450.050.9705748
TTCTGCT4500.050.84327752
GTCTTCT4450.050.62968449
Produced by FastQC (version 0.11.5)