FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204257
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43052.1076389058881704No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7040.3446638303705626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6290.3079453825327896No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6010.29423716200668765No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5130.2511541832103673No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT5050.24723754877433823No Hit
GTATCAACGCAGAGTACGGGAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTC4220.20660246650053607No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4050.19827961832397423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.1884880322339014No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGA3730.18261308057985773No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.15715495674566846No Hit
AAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGG3000.146873791351092No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.14344673621956652No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.14148841900155198No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.13610304665201192No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.13414472943399736No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.12973851569346462No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2540.12435314334392457No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.12386356403942093No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT2380.11651987447186633No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC2360.11554071586285905No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.11554071586285905No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.1126032400358372No Hit
GTATCAACGCAGAGTACGGGATGGTGACCACGGGTGACGGGGAATCAGGG2300.1126032400358372No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC2280.11162408142682992No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.11113450212232628No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2260.11064492281782265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.110155343513319No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGG2210.10819702629530444No Hit
CAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCC2130.10428039185927532No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCG2130.10428039185927532No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2100.1028116539457644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.1013429160322535No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.1013429160322535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTGT156.7740405E-494.3965466
CGGTTCA309.468931E-486.7247370
TCGTCAA309.468931E-486.7247370
CCCTATG150.00406787960.0957372
TTGCTCC200.0047996257.6309548
GATTGCC250.00576030955.02937364
GACAAGG250.00678479252.79978661
GACTCAG300.001176150740.5202225
CGGGACT1001.8189894E-1236.0839517
GGGACTC608.480838E-735.09021418
ACAGGGT350.002644581734.357251
ATAAGAG350.002650944334.3404243
GGACTCC551.8819397E-532.81967519
CGACGCA450.00433379131.0593539
GACTCCC603.3992466E-530.0994620
GTCACTA802.3691973E-730.0478732
ACCGTGC400.005097571830.0478738
TACTGGA504.1751185E-430.0478692
GCCTTTC450.00680986328.31787131
GTATCAA34550.027.6697671
Produced by FastQC (version 0.11.5)