FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179398
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGCTAGTTTACCATTCCCTTTCGGCCCTGGGA60710.5147541372802057No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41140.34882202615232516No Hit
CAGTAATACACTGCAAAATCTTCAGGTTCTAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA38050.3226222191321335No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34580.2932004293715947No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGTAA33420.28336490311158746No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29290.2483470380651824No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26750.22681062711654587No Hit
GTGTATTACTGTCAGCAGTATGCTAGTTTACCATTCCCTTTCGGCCCTGGGACCAAAGTGGATATCACACGAA25240.21400748517463997No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24510.20781788675239402No Hit
CATCTATGGTGCATCCCGCAGGGCCACTGGCATCCCAGACAGGTTCGGTGGCAGTGGGTCTGGGACAGACTTCA23280.19738883735600704No Hit
CTCCTGCAGGGCCAGTCAGACTGTCAACGGCAACTACTTGGCCTGGTACCAGCAGAAACCTGGTCAGGCTCCCAG22800.19331896442083166No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22270.18882514638824213No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22210.1883164122713452No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTAGAACCTGAAGATT22090.18729894403755137No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21490.1822116028685821No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA19920.16889972680977922No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19720.16720394642012282No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19270.16338844054339588No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19170.16254055034856768No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18160.15397685938080274No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17840.15126361075735248No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17560.1488895182118335No Hit
GTAATACACTGCAAAATCTTCAGGTTCTAGTCTGCTGATGGTGAGAGTGA17200.14583711351045192No Hit
GTGCAGCCACAGTTCGTGTGATATCCACTTTGGTCCCAGGGCCGAAAGGG16490.13981709312717167No Hit
GTCCCAGACCCACTGCCACCGAACCTGTCTGGGATGCCAGTGGCCCTGCG16240.13769736764010115No Hit
GTATGCTAGTTTACCATTCCCTTTCGGCCCTGGGACCAAAGTGGATATCACACGAACTGTGGCTGCACCAT16040.13600158725044473No Hit
CCATAGATGAGGAGGCTGGGAGCCTGACCAGGTTTCTGCTGGTACCAGGCCAAGTAGTTGCCGTTGACAGTCTGA15610.1323556594126834No Hit
GTCTATTACTGTGCGAGACGTTATTACGGTACCTACTTGTCGGGTTTGGA15440.13091424608147548No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15240.12921846569181905No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15210.12896409863337058No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATATCCACTTTGGTCC15120.12820099745802518No Hit
GACTTCACTCTCACCATCAGCAGACTAGAACCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGCTAGTTTA15030.12743789628267982No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14870.12608127197095467No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14760.12514859275664364No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14610.12387675746440134No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14050.11912857237336336No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13730.11641532374991309No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA13710.11624574571094744No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13710.11624574571094744No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13650.11573701159405053No Hit
GCCACAGTTCGTGTGATATCCACTTTGGTCCCAGGGCCGAAAGGGAATGG13360.11327813002904871No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13340.11310855199008307No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13120.11124319356146102No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13030.11048009238611564No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACCGAACCTGTCTGGGATGCCA12910.10946262415232177No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12730.107936421801631No Hit
GCAGAAACCTGGTCAGGCTCCCAGCCTCCTCATCTATGGTGCATCCCGCAGGGCCACTGGCATCCCAGACAGGTT12610.10691895356783716No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12370.10488401710024944No Hit
CTAGCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGTTCTAGTCTGCTGATGGTGAGAGTGAAGTCTGTC12130.10284908063266174No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12120.10276429161317892No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12110.1026795025936961No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12060.10225555749628201No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTT150.002516137267.86188523
CGCGTAT252.6156023E-454.99165339
CTACGCT2200.052.51000228
ACTACGC2200.052.4832527
TACGCTG2250.048.3537429
GGTATCA12100.045.9831921
ACGAGAC2450.045.6990222
ACGCTGC2400.045.3663430
CTTATAC2600.044.3655851
ATCTCCG2650.043.5100410
GCGTATG405.0658433E-543.03249440
CGAGACT2700.041.47115323
CCCACGA2850.040.4618519
CACGAGA2800.039.98325321
AACTGTG25950.039.85064370
TCTCCGA2850.039.26680411
GTATCAA30400.038.8370551
TGCATCG1350.037.93113734
CGCTGCA2800.037.7119731
GAGACTA3000.037.32878524
Produced by FastQC (version 0.11.5)