FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179398
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGCTAGTTTACCATTCCCTTTCGGCCCTGGGA55360.46939201185689655No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40870.34653272262628904No Hit
CAGTAATACACTGCAAAATCTTCAGGTTCTAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA36470.30922555405384783No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGTAA30750.2607262349096743No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28460.24130954944810828No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28390.2407160263117285No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28100.2382571447467267No Hit
GTGTATTACTGTCAGCAGTATGCTAGTTTACCATTCCCTTTCGGCCCTGGGACCAAAGTGGATATCACACGAA26050.22087539575274842No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25130.21307480596032893No Hit
CTCCTGCAGGGCCAGTCAGACTGTCAACGGCAACTACTTGGCCTGGTACCAGCAGAAACCTGGTCAGGCTCCCAG22260.18874035736875933No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTAGAACCTGAAGATT22140.18772288913496546No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21760.18450090639461827No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21040.17839609699185519No Hit
CATCTATGGTGCATCCCGCAGGGCCACTGGCATCCCAGACAGGTTCGGTGGCAGTGGGTCTGGGACAGACTTCA20920.17737862875806132No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20330.17237607660857487No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA19720.16720394642012282No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19320.16381238564081No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18800.15940335662770327No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17560.1488895182118335No Hit
GTCCCAGACCCACTGCCACCGAACCTGTCTGGGATGCCAGTGGCCCTGCG17350.14710894880269426No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16900.14329344292596732No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16710.14168245155579373No Hit
GTGCAGCCACAGTTCGTGTGATATCCACTTTGGTCCCAGGGCCGAAAGGG16480.13973230410768883No Hit
GTCTATTACTGTGCGAGACGTTATTACGGTACCTACTTGTCGGGTTTGGA16430.13930835901027472No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16280.1380365237180324No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16150.13693426646475573No Hit
GTAATACACTGCAAAATCTTCAGGTTCTAGTCTGCTGATGGTGAGAGTGA16130.1367646884257901No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15910.13489932999716805No Hit
GACTTCACTCTCACCATCAGCAGACTAGAACCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGCTAGTTTA15830.1342210178413055No Hit
GTATGCTAGTTTACCATTCCCTTTCGGCCCTGGGACCAAAGTGGATATCACACGAACTGTGGCTGCACCAT15410.130659879023027No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15060.12769226334112826No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14950.12675958412681723No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14780.12531817079560928No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14690.1245550696202639No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATATCCACTTTGGTCC14280.12107871982146824No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14230.12065477472405414No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA14000.11870462727594924No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13970.1184502602175008No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13720.11633053473043026No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACCGAACCTGTCTGGGATGCCA13660.11582180061353335No Hit
GCCACAGTTCGTGTGATATCCACTTTGGTCCCAGGGCCGAAAGGGAATGG13540.1148043323797395No Hit
CCATAGATGAGGAGGCTGGGAGCCTGACCAGGTTTCTGCTGGTACCAGGCCAAGTAGTTGCCGTTGACAGTCTGA13290.11268460689266899No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12510.10607106337300894No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12400.1051383841586979No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12160.10310344769111021No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12070.10234034651576483No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12020.10191640141835072No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12020.10191640141835072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC500.067.780633
CCTAACG653.6379788E-1252.1389432
GGTATCA11050.043.5679861
GTATCAA31300.043.218521
TATCAAC37500.036.0592962
AACTGTG24400.035.4371370
TCAACGC38550.035.0771334
ATCAACG38600.035.03173
CTAGTGA8500.034.94368769
CAACGCA39150.034.5395555
AACGCAG39350.034.2778786
ACGCAGA40850.033.1021657
CGCAGAG41500.032.7470258
CGGAAAT1057.421477E-1032.4194730
AGAGTAC41750.032.14506511
CGGGACT951.0410076E-832.1243217
TCGCATT851.4058423E-731.91571218
GCAGAGT42650.031.6256629
TTCGATC1101.1004886E-931.2520339
CAGAGTA46650.029.0592210
Produced by FastQC (version 0.11.5)