FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927921
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20740.22351040659711335No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18940.2041122035173253No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18560.20001702731159227No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16880.18191203777045675No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15900.17135079387146104No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11970.1289980504805905No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11750.12662715899306082No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG11730.1264116234032854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11380.12263975058221552No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10880.11725136083782994No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10100.10884547283658845No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9960.1073367237081605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG750.068.5490536
GTATCGC800.064.20524635
TGTACGC200.00784396150.92056726
CGCGTAT352.3020953E-549.17803239
AGTATCG1500.047.86433834
GGTATCA21500.047.6169241
GTGGTAT4850.041.2385251
ACCGATC4750.040.75185427
TGGTATC4750.039.9527632
GACCGAT5100.039.2720726
GAGACCG5200.039.13586424
CGATCAG5000.038.77917529
GTATCAA54900.038.777531
AGACCGA5200.038.49190525
ACGAGAC5450.037.95889322
TATACAC5900.037.3368033
CCCACGA5500.036.36488319
TATCTCG1800.036.17866536
CGTATGC1151.8189894E-1236.08926841
CGAGCCC5550.036.02367415
Produced by FastQC (version 0.11.5)