FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501548
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56320.3750795845354261No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45010.2997573171154036No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA40760.2714531936374995No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGGCTGAAGATT38590.25700144117936957No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36600.24374845159795094No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35470.23622288464970817No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG34260.22816453420070487No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29790.1983952560957092No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29570.19693010146861772No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT27440.18274474076086814No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27010.17988102944428017No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA26890.17908185419313935No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC26710.1778830913164281No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGAGGCCAGTGGCCCTGTT26480.17635133875174153No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25210.16789340067716782No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24980.16636164811248125No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24840.16542927698615031No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACA24810.16522948317336508No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24640.16409731823424892No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGGCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG24130.16070082341690042No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24040.1601014419785448No Hit
GGCCAAAAGTGTACGGAGGCCAGTTGCTACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGCCTCTAGGCTG24020.15996824610335467No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGCCTGGTA24000.15983505022816455No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGTACACTTTTGGCCA23690.15777051416271742No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22720.15131051421599576No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGGCCAGTTGCTACGCTG22490.14977876165130918No Hit
GCCTAGAGGCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC21950.14618247302117549No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21690.1444509266437037No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC21620.14398474108053821No Hit
TCTATGATGCATCCAACAGGGCCACTGGCCTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT21460.1429191740790171No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21160.14092123595116507No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20850.13885669988571794No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20280.13506061744279904No Hit
GTAATAAACTGCAAAATCTTCAGCCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20230.1347276277548237No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGG19900.13252989581418642No Hit
GTCAGAGTGTTGGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG19780.1317307205630456No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19470.12966618449759848No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19460.1295995865600034No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC19090.1271354628689859No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18750.12487113299075353No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGAGGCCA18630.12407195773961271No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG18340.12214061754935573No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCCTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA17770.11834453510643683No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17670.11767855573048613No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT17540.11681278254175025No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17220.11468164853870805No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17110.1139490712251623No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16870.11235072072288066No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16840.11215092691009546No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16590.11048597847021872No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16210.10795525684160613No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16080.10708948365287024No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA15650.1042257723362823No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15560.10362639089792668No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15560.10362639089792668No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15400.10256082389640557No Hit
CAACAGGGCCACTGGCCTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG15310.10196144245804994No Hit
ACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC15150.10089587545652887No Hit
GCGTAGCAACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT15130.10076267958133872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT453.0646333E-853.5878439
GGTATCA13850.044.1210631
AATCGCG803.6379788E-1142.95337736
CGAGACG2600.042.0234523
ATCGCGT758.9858077E-1041.2739937
GCGTATG602.918805E-740.23686640
CGCTCAT2950.039.4089228
GACGCTC2800.039.04518526
ACGAGAC2850.038.33207322
ATCTCCG3050.036.92798210
GTATCAA36250.036.0689471
TTATACC952.4556357E-1035.931534
TATACAC3350.035.6621973
GAGACGC3100.035.24547224
CACGAGA3100.035.239621
CCCACGA3200.035.19932619
CATGAAT3350.034.78577432
CGTATGC1003.783498E-1034.5305841
CGTATGG709.671548E-734.53057541
TGAATCG1201.8189894E-1234.31093634
Produced by FastQC (version 0.11.5)