FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501548
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59770.39805587300572476No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA37420.2492094824807465No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37030.24661216291453886No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGGCTGAAGATT36390.2423498949084545No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36100.24041855471819748No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33350.22210412187955364No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32210.21451195699371584No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31670.2109156683635821No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG30960.20618721479433227No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA27270.18161257582175197No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGAGGCCAGTGGCCCTGTT27190.1810797923209914No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT26770.17828267894199853No Hit
GGCCAAAAGTGTACGGAGGCCAGTTGCTACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGCCTCTAGGCTG26270.1749527820622451No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGGCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG25170.16762700892678756No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGCCTGGTA25000.1664948439876714No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGGCCAGTTGCTACGCTG24900.1658288646117207No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24870.1656290707989355No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24680.16436370998462918No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24070.16030123579133002No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24050.16016803991613987No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC23870.15896927703942865No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGTACACTTTTGGCCA23730.15803690591309769No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23270.1549734007837245No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22560.15024494721447465No Hit
GTAATAAACTGCAAAATCTTCAGCCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21920.14598267920839028No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21870.14564968952041493No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC21210.14125422563914042No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACA21150.14085463801356998No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21150.14085463801356998No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20890.1391230916360982No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20890.1391230916360982No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20660.13759133907141163No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGG20300.13519381331798916No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20170.13432804012925326No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGAGGCCA20140.13412824631646808No Hit
GCCTAGAGGCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC20050.13352886487811244No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19980.13306267931494697No Hit
TCTATGATGCATCCAACAGGGCCACTGGCCTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT19590.1304653597487393No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19170.1276682463697464No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG18920.12600329792986972No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC18450.12287319486290148No Hit
GTCAGAGTGTTGGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG18240.12147463817340505No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18230.12140804023580998No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18020.12000948354631354No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16480.10975340115667298No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT16380.1090874217807223No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16010.10662329808970476No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16010.10662329808970476No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15920.10602391665134915No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCCTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA15860.10562432902577873No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15720.10469195789944777No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15710.1046253599618527No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15490.10316020533476118No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15160.10096247339412394No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15160.10096247339412394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12050.043.3021051
GTATCAA35000.038.2938581
CCGCTAT750.00162092737.97364870
TATCAAC38500.034.531892
TCGATCC400.00275681934.08122612
ATCAACG38900.033.995883
TCAACGC39400.033.3914454
CAACGCA39550.033.2648055
AACGCAG40450.033.11566
AATGGTA8050.031.84125970
ACGCAGA42900.031.2233437
CGCAGAG42600.030.9631778
AGAGTAC42850.030.70298611
ACGTAGG1251.4006218E-1030.06157527
TTAATCG1151.7626007E-929.9058137
GCAGAGT44600.029.5746929
TCCGGTC1302.2737368E-1028.8398828
CTCGCTA854.8293423E-628.09777823
CCGGTCT1603.6379788E-1227.6928429
GTGGTGA8450.026.9635470
Produced by FastQC (version 0.11.5)