FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481905
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38350.25878851883217885No Hit
GTATATTACTGTGCGAGAGACTACGGTGGTAACTGGAACTGGGGCCAGGG37510.25312013928018323No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36720.24778916327294936No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28580.19285986618575415No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA26580.1793637243952885No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26340.17774418738043263No Hit
GTAATATACGGCCGTGTCTTCAGATCTCAGGCTGTGCAGTTCCATGTAGCCTGTGCTCGTAAATCTGTCCGCG26220.17693441887300468No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25820.17423519051491154No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25330.17092863577624745No Hit
CATCTATGCTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA24460.1650578140973949No Hit
GTATAGGAGATTACGCTCCGAAGTTCCAGGGCAGAGTCACCATCAGCGCG24190.16323583495568206No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGACTTTGCAGTGTATTTCTGTCAGCAGTA24070.1624260664482541No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23440.15817478178425742No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22610.15257388294121418No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAACTACTTAGCCTGGTACC22400.1511567880532153No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21990.14839007898616982No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21390.14434123644903013No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20930.14123712383722303No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20920.1411696431282707No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20240.1365809549195124No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20210.1363785127926554No Hit
GAACTGCACAGCCTGAGATCTGAAGACACGGCCGTATATTACTGTGCGAG20130.13583866712103676No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20080.13550126357627512No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20040.1352313407404658No Hit
ACATGGAACTGCACAGCCTGAGATCTGAAGACACGGCCGTATATTACTGT19610.1323296702555157No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT19560.13199226671075406No Hit
GTCTTTGTCCCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA19480.13145242103913543No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19310.13030524898694587No Hit
GTCTCCAGGCACCCTGTCTTTGTCCCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG19240.12983288402427956No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18890.12747105921094806No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAAGAATCATCCCTTTCGTTGGT18700.12618892574085383No Hit
CTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCA18640.12578404148713987No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18560.12524419581552124No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18030.12166771824104784No Hit
GGCCAAAAGTGTACGGTGAGCTACCGTACTGCTGACAGAAATACACTGCA18020.12160023753209552No Hit
GTACGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCAT17990.12139779540523853No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17910.1208579497336199No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17750.11977825839038264No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG17690.11937337413666868No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17160.11579689656219527No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17000.11471720521895802No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16780.11323262962200681No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16780.11323262962200681No Hit
GCATAGATGAGGAGCCTGGGAGGCTGGCCAAGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTGCCAACACTCTGA16250.1096561520475334No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16150.10898134495801012No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16130.10884638354010548No Hit
GTATTTCTGTCAGCAGTACGGTAGCTCACCGTACACTTTTGGCCAGGGGA16110.10871142212220081No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16070.10844149928639152No Hit
GTGCAGTTCCATGTAGCCTGTGCTCGTAAATCTGTCCGCGCTGATGGTGA16040.10823905715953452No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGACTTTGCAGTGTATTTCTGTCAGCAGTACGGTAGCTCA16040.10823905715953452No Hit
GTGTATTTCTGTCAGCAGTACGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAA16000.10796913432372521No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15980.10783417290582055No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTG15950.10763173077896356No Hit
CTCCAGGCACCCTGTCTTTGTCCCCAGGGGAAAGAGCCACCCTCTCCTGC15870.10709188510734495No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15860.1070244043983926No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15630.10547234809248907No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15580.10513494454772743No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCC15260.10297556186125291No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15210.10263815831649127No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTCCCCTGGCCCCAGTTCCAGTTACCACCGTAGT14850.10020885279420745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)