FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481905
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38980.2630398034961755No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38120.2572364625262753No Hit
GTATATTACTGTGCGAGAGACTACGGTGGTAACTGGAACTGGGGCCAGGG35800.24158093804933514No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32520.21944726551297147No Hit
GTAATATACGGCCGTGTCTTCAGATCTCAGGCTGTGCAGTTCCATGTAGCCTGTGCTCGTAAATCTGTCCGCG31220.2106747733491688No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26670.17997105077585945No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA25320.17086115506729513No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23700.15992928021701797No Hit
CATCTATGCTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA23610.159321953836447No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23540.15884958887378073No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGACTTTGCAGTGTATTTCTGTCAGCAGTA22840.15412593924711773No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22660.15291128648597582No Hit
GTATAGGAGATTACGCTCCGAAGTTCCAGGGCAGAGTCACCATCAGCGCG22290.15041450025473968No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAACTACTTAGCCTGGTACC22110.14919984749359777No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22110.14919984749359777No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21640.14602825417283832No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21280.14359894865055453No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21270.1435314679416022No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21180.14292414156103123No Hit
GTCTTTGTCCCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA19480.13145242103913543No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19300.13023776827799352No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18760.12659380999456782No Hit
GTCTCCAGGCACCCTGTCTTTGTCCCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG18630.12571656077818755No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18520.12497427297971193No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18470.1246368694349503No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT18270.12328725525590373No Hit
GAACTGCACAGCCTGAGATCTGAAGACACGGCCGTATATTACTGTGCGAG18080.12200512178580948No Hit
CTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCA17510.11815872137552676No Hit
ACATGGAACTGCACAGCCTGAGATCTGAAGACACGGCCGTATATTACTGT17440.11768635641286047No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17100.11539201230848131No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16980.11458224380105338No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16950.11437980167419638No Hit
GTACGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCAT16850.1137049945846731No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16850.1137049945846731No Hit
GTGCAGTTCCATGTAGCCTGTGCTCGTAAATCTGTCCGCGCTGATGGTGA16830.11357003316676845No Hit
GGCCAAAAGTGTACGGTGAGCTACCGTACTGCTGACAGAAATACACTGCA16750.11303018749514981No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16690.11262530324143585No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16540.11161309260715094No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16400.11066836268181833No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16330.11019599771915205No Hit
GTATTTCTGTCAGCAGTACGGTAGCTCACCGTACACTTTTGGCCAGGGGA16250.1096561520475334No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTG16040.10823905715953452No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16020.10810409574162987No Hit
GTGTATTTCTGTCAGCAGTACGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAA15970.10776669219686823No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15890.1072268465252496No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG15290.1031780039881099No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGACTTTGCAGTGTATTTCTGTCAGCAGTACGGTAGCTCA15290.1031780039881099No Hit
GCATAGATGAGGAGCCTGGGAGGCTGGCCAAGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTGCCAACACTCTGA15250.10290808115230059No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15130.10209831264487265No Hit
CTCCAGGCACCCTGTCTTTGTCCCCAGGGGAAAGAGCCACCCTCTCCTGC15110.10196335122696798No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15090.10182838980906334No Hit
CTCCTATACCAACGAAAGGGATGATTCTTCCCATCCACTCAAGCCCTTGT15070.10169342839115866No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAAGAATCATCCCTTTCGTTGGT15010.1012885441374447No Hit
GTTCCATGTAGCCTGTGCTCGTAAATCTGTCCGCGCTGATGGTGACTCTGCCCTGGAACTTCGGAGCGTAATCTC14980.10108610201058774No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT553.0651697E-453.25044370
GCGCGTA351.9839803E-550.4327555
GGTATCA12150.044.4153481
CGCGTAA404.3088978E-544.2296656
CGCGCGG1451.765693E-840.3968970
GTACGTA705.974316E-638.82401369
GTATCAA31350.038.4328881
TCGTTAA400.002261287535.4861957
TCAGCAT16850.032.5902270
TATCAAC36850.032.498162
AACGGGT702.7143446E-432.35334469
AACGCAG37350.032.2382436
TCAACGC37550.032.0654534
ATCAACG37500.032.0210043
CAACGCA37850.031.7216935
ACGCAGA40450.029.8514257
CGCAGAG40900.029.2741998
AGAGTAC41600.028.94369511
AATATAC20550.027.7306733
GCGTAAA657.3438295E-427.2970757
Produced by FastQC (version 0.11.5)