FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206851
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32730.2712016644971086No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27670.229274367755423No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGTACCCCAGTGACACTACCTGCATATGAG25330.20988506451914943No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24100.19969325127956972No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGTACCCCAGTGACA23840.19753888425331712No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23780.1970417226318742No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22920.18991573939119244No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21610.17906104398968886No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20180.16721202534529944No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTGTCACTGGGGTACTATTCGG19780.16389761453568005No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18820.15594302859259346No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18040.1494799275138356No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17780.14732556048758297No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCACCATCTCCTG17540.14533691400181134No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG16980.14069673886834413No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16970.14061387859810368No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAACCTTGTCTCCTGGTACCAACAGCACCCAAACAAAGC16420.13605656373487696No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16380.135725122653915No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16180.1340679172491053No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15690.13000776400732153No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15080.12495328752265193No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15070.12487042725241145No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14990.12420754509048755No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14690.121721736983273No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAATAGTACCCCAGTGACAC14620.12114171509158961No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14090.11675012076884388No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14020.11617009887716048No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13830.11459575374259126No Hit
CTTATAACCTTGTCTCCTGGTACCAACAGCACCCAAACAAAGCCCCCAAA13830.11459575374259126No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13580.11252424698657913No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGGCAGTCGATCAC12960.10738691023166903No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12810.10614400617806176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT352.3442097E-549.0289839
TCTATCG658.277942E-443.53180370
CTACGCT1600.042.15989328
ACTACGC1600.042.11960627
GGTATCA17400.040.7524831
CTAACGC702.761226E-838.4040373
TACGCTG1850.038.3287829
GTAGTTT4550.037.31297770
GTATCAA40650.036.78921
TCGCGTA501.9448966E-434.24347738
CCTAACG808.94197E-833.603532
CGTAATT1102.8128095E-432.15417570
GTCACCG3400.031.20846470
GACTACG2100.030.45999126
TATCAAC49800.030.1622052
ATCAACG50200.029.921873
TCAACGC50650.029.5896844
CAACGCA51800.028.997645
ACGCTGC2450.028.986830
AACGCAG52250.028.8765286
Produced by FastQC (version 0.11.5)