FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597811
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20160.3372303286490212No Hit
GTATAATAATTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT19060.3188298642882115No Hit
TTATTATACTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATTGTGAGAGTGAATTCTGTC17890.2992584612862593No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16020.2679776718728829No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13020.21779458725249284No Hit
ATAATATACAGCCGTATCCTCGGCTCTCAGGCCATTCATTTGAAGATACA11240.18801929037772808No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10750.17982271988973103No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10660.17831722735111932No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10010.16744422568336817No Hit
GTATATTATTGTGCGGTATACGATGCTAAGTCGGAGTGGGATGGCCACTGGGGCCAGGGAATCCTGGTCACCGT9950.16644056399096036No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9440.15790943960549406No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8990.15038197691243554No Hit
CTCCTGTGCAGCCTCTGGATTCACCGTCAATTCCGAGCACATGAGCTGGTTCCGCCAGGCTCCAGGGAAGGGG8770.1467018840402736No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8550.14302179116811164No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8310.13900714439848044No Hit
GTGGATGCACCATACAGCAGGCGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCCTAAATCAATTCTAATG8140.13616343626999167No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGA7940.13281789729529903No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAATTGATTTAGGCTGGTATCAGCAGAAA7920.13248334339782974No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7810.13064329696174878No Hit
CCATACAGCAGGCGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCC7750.129639635269341No Hit
GCTGTATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA7730.12930508137187172No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7680.12846869662819854No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT7480.12512315765350587No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7280.1217776186788132No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7250.12127578783260931No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT7220.12077395698640539No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC7130.1192684644477937No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7090.11859935665285518No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7020.11742841801171275No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG6990.11692658716550883No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT6980.11675931021677419No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6750.11291194039587762No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6560.1097336783699196No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6510.10889729362624642No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6450.10789363193383862No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6390.10688997024143082No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6170.10320987736926888No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6150.10287532347179962No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG6110.10220621567686108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18000.037.7396351
GTGGTAT2500.036.6829221
AGCTTAG800.002148687835.8476570
TATACCT400.0027891333.99698326
CTATACC400.00279142333.99128725
CCTATAC400.002796013133.97989724
ATCAACG19850.033.851773
TATCAAC20100.033.439122
TCAACGC19950.033.3390774
CAACGCA20400.032.606385
AACGCAG20600.032.454566
CGCAGAG21600.031.4233848
ACGGGGC1209.276846E-1131.11956416
ACGCAGA21550.031.0238487
AGAGTAC22150.030.79633311
ACGGAAC603.7386155E-430.63096466
AACGGAA603.9937394E-430.28790965
ATTAGGG450.004979348730.20182221
ATTGCAC450.005007970630.1664493
GCAGAGT22400.030.1496149
Produced by FastQC (version 0.11.5)