FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694341
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22850.32908902110058313No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22750.3276488065662261No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20230.2913554003004287No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16120.23216258293835448No Hit
GATTATTACTGCAGCTCATATACACGCAGCAGCGCGAGGGTCTTCGGCGGGGGGACCAAGCTGACCGTCCTAGGT15940.22957019677651183No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAAGACCCTCGCGCTGCTGCGTGTATATGAGCTG15410.22193705974441952No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15060.21689630887416989No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG14780.2128637081779702No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAAGACCCTCGCGCTGCTGCGTGTATATGAGCTGCAGTAATAAT14610.21041534346956325No Hit
CATATACACGCAGCAGCGCGAGGGTCTTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCC14510.20897512893520617No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14360.20681480713367062No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCGCTGGAACCAGCAGTGACATT14310.20609469986649212No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCC14150.20379035661152087No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT13500.1944289621382001No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13330.19198059742979315No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12860.18521158911831506No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12430.1790186666205798No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12260.17657030191217282No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12260.17657030191217282No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11590.16692086453198068No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11410.164328478370138No Hit
GTCGATCACCATCTCCTGCGCTGGAACCAGCAGTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACA11200.16130402784798825No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11170.16087196348768112No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10920.15727142715178852No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10860.15640729843117432No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10860.15640729843117432No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG10820.1558312126174315No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10540.15179861192123179No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10230.14733394686472495No Hit
GTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGG9930.14301330326165385No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9690.13955678837919697No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9660.13912472401888984No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8890.12803507210434065No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC8870.12774702919746928No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAACCCCCCAAACTCATGATTTATGAGGTCAGTAAT8680.1250106215821909No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT8530.12285029978065533No Hit
GTAATATACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATATA8440.12155410669973399No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT8350.12025791361881266No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8200.1180975918172771No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT8110.11680139873635577No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8050.11593727001574154No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7750.11161662641267042No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7730.11132858350579904No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7600.10945630461113487No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7450.10729598280959932No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC7430.10700793990272792No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7360.10599978972867799No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7290.10499163955462805No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGGCAGACTTGGAGCCAGAGAAG7230.10412751083401384No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7210.10383946792714242No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7130.1026872962996568No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7110.10239925339278538No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC7020.10110306031186406No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT200.007396785551.68084339
GCGAAAC305.855395E-446.70264452
GGTATCA7700.046.096031
GTATCAA23550.039.5633131
ACTCGCG350.001362155439.34155338
GTGCGAG350.001422546838.99590711
CGTATGC1900.038.161141
TCGTATG2000.037.9351540
TGGTATC2100.037.3764572
TATCAAC25600.036.3925322
TCAACGC26700.034.8932154
ATCAACG26800.034.7630163
TATACAG2950.034.7047465
TCTCGTA1900.034.4238638
CAACGCA27200.034.2517975
CCGTCTT2250.034.02224347
GTATGCC2150.033.7655942
AACGCAG28000.033.5169336
CGAGACG2250.033.37758623
ATCTCGT2000.032.66232337
Produced by FastQC (version 0.11.5)