FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636758
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTACAGTGCCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAAGGAACTGTGGCTGCACCAT82240.5024566857165201No Hit
GATCTATACTGCATCCAGTTTGCAAAATGGGGTCCCATCAAGGCTCAGTGGCAGTGGATCTGGGACAGATTTCA80420.4913371433040193No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT72550.4432542868279855No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC67240.4108121053937112No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60760.3712216466942578No Hit
GTATAGATCATGAGTCTTGGGGCTTTCCCTGGTTTCTGCTGATACCAGTT57570.35173189927894044No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC50940.3112249947762589No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43230.2641196804903352No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC42920.2622256924969971No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG40310.24627953552082837No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39860.24353019811114412No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG39630.24212498121286102No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGGACATTAGCAGCTTTTTAAACTGGTATCAGCAGAAA39380.24059757154081424No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35800.21872506503710382No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33560.20503947437556438No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG33290.20338987192975383No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32340.19758571517597595No Hit
ATACTGCATCCAGTTTGCAAAATGGGGTCCCATCAAGGCTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA32190.1966692693727478No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGGACATTAGCAG32120.19624159466457475No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31980.1953862452482285No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31520.19257581145166236No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31230.19080401623208806No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC29200.17840144969506794No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28810.17601869060667488No Hit
AGTCAGGACATTAGCAGCTTTTTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCAAGACTCATGATCTATA28290.17284167848881754No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28160.1720474254593532No Hit
CATCAAGGCTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG27150.16587669038428404No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGGGCA26820.16386050961718227No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT26740.16337173852212727No Hit
ACTTACTACTGTCAACAGAGTTACAGTGCCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAAGGAA26320.1608056902730886No Hit
GTCTTGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAAAAGCTGCTA26200.16007253363050616No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25960.15860622034534122No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT25840.15787306370275875No Hit
GTGCAGCCACAGTTCCTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTC25470.15561249738812946No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25350.154879340745547No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24510.14974724424746969No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCC23920.14614255742143922No Hit
ACAGTGCCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAAGGA23580.14406528026745555No Hit
GCCTTGATGGGACCCCATTTTGCAAACTGGATGCAGTATAGATCATGAGTCTTGGGGCTTTCCCTGGTTTCTG23340.1425989669822906No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23010.1405827862151888No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA22560.13783344880550452No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22240.13587836442528461No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22110.13508411139582027No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21670.1323958703730179No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21540.13160161734355355No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21450.1310517498616167No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAGCCTTGATGGGACCCCA20790.12701938832741308No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGG20630.12604184613730313No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19860.12133742434739894No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19770.1207875568654621No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19360.11828260500330531No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18860.11522778565921168No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18710.1143113398559836No Hit
ATGCAGTATAGATCATGAGTCTTGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAAAAGCTGCTAATGTC18630.11382256876092862No Hit
GCCCCAAGACTCATGATCTATACTGCATCCAGTTTGCAAAATGGGGTCCCATCAAGGCTCAGTGGCAGTGGAT18530.11321160489210991No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18520.11315050850522802No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18490.1129672193445824No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC18400.11241735186264554No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18260.11156200244629934No Hit
GGGCACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT17910.10942362890543379No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17900.10936253251855191No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17620.10765183368585948No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17330.10588003846628519No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCCCCGTGGACGTT17210.1051468818237027No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17180.10496359266305708No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17120.10459701434176587No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCCCCGTGGACGTTCGGCCAAGGGA16990.10380276131230153No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT16980.10374166492541964No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16910.10331399021724653No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16890.10319179744348278No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC16800.10264192996154593No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16800.10264192996154593No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCCTCAGGGAGA16560.10117561667638099No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16510.10087013474197164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG2750.043.33239423
CGCTCAT2950.039.30656428
ACGAGAC3150.038.90477822
CACGAGA3150.037.82177721
GAGACGC3100.037.3485924
TCGTATG754.062713E-836.75831240
GACGCTC3250.036.6984126
GTATCAA42400.036.6765751
CTTATAC3250.036.6457061
CCCACGA3450.034.5255419
CGTATGC905.1932147E-934.4993541
TGAATCG1004.129106E-1034.2447534
TATCAAC45900.033.5833442
GTGTACG450.003184147933.09370467
ATCTCCG3850.031.81173110
ATCAACG48750.031.6907793
GTGGTAT4100.031.5382921
TCAACGC49100.031.3946134
AGACGCT3800.031.37431525
TATACAC4350.031.2912143
Produced by FastQC (version 0.11.5)