FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004692244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636758
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTACAGTGCCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAAGGAACTGTGGCTGCACCAT80670.49286455297606613No Hit
GATCTATACTGCATCCAGTTTGCAAAATGGGGTCCCATCAAGGCTCAGTGGCAGTGGATCTGGGACAGATTTCA78050.47685729961301543No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76750.46891476931837206No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC68170.4164940693737254No Hit
GTATAGATCATGAGTCTTGGGGCTTTCCCTGGTTTCTGCTGATACCAGTT58510.3574749596458365No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50590.3090866212353934No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48730.29772269327536505No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46610.2847702592564081No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43200.26393639132968955No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG40250.24591295719953712No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC39200.23949783657694051No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGGACATTAGCAGCTTTTTAAACTGGTATCAGCAGAAA37620.22984460744960464No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34670.21182117331945224No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33340.20369535386416318No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGGACATTAGCAG32730.19996847426436895No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32570.19899093207425897No Hit
ATACTGCATCCAGTTTGCAAAATGGGGTCCCATCAAGGCTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA32150.19642488382522033No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31570.19288129338607174No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31170.19043743791079681No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31080.18988757042885998No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29690.1813951726522797No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC28270.1727194857150538No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27720.1693591844365508No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG27660.16899260611525957No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27530.16819835308579523No Hit
AGTCAGGACATTAGCAGCTTTTTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCAAGACTCATGATCTATA26710.16318844936148166No Hit
GTCTTGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAAAAGCTGCTA26660.1628829674270723No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26470.16172213607631672No Hit
GTGCAGCCACAGTTCCTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTC26350.16098897943373425No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26100.15946156976168743No Hit
CATCAAGGCTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG25580.15628455764383006No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGGGCA25550.15610126848318445No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT25360.15494043713242886No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25120.15347412384726392No Hit
ACTTACTACTGTCAACAGAGTTACAGTGCCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAAGGAA24550.14999162979499717No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCC24400.14907518399176908No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA24090.14718119599843105No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24020.14675352129025793No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23960.1463869429689667No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT23880.14589817187391174No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23810.14547049716573862No Hit
GCCTTGATGGGACCCCATTTTGCAAACTGGATGCAGTATAGATCATGAGTCTTGGGGCTTTCCCTGGTTTCTG23310.142415677821645No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22430.13703919577604018No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22230.13581726803840274No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAGCCTTGATGGGACCCCA21230.1297076293502155No Hit
ACAGTGCCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAAGGA21200.12952434018956988No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGG21040.12854679799945992No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20180.12329250872761886No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20120.12292593040632763No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19930.12176509905557206No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19630.11993220744911587No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19510.11919905080653341No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19230.11748835197384097No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18950.11577765314114855No Hit
GGGCACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT18740.11449462901662923No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18560.1133948940527555No Hit
ATGCAGTATAGATCATGAGTCTTGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAAAAGCTGCTAATGTC18170.11101213496436248No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17960.10972911083984316No Hit
GCCCCAAGACTCATGATCTATACTGCATCCAGTTTGCAAAATGGGGTCCCATCAAGGCTCAGTGGCAGTGGAT17900.10936253251855191No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC17640.10777402645962322No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17620.10765183368585948No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCCTCAGGGAGA17490.10685758065639514No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17400.10630771317445828No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCCCCGTGGACGTT17240.10533017098434833No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17220.10520797821058459No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16780.10251973718778219No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16760.10239754441401844No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16710.10209206247960907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)