FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590510
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTACTGGACATCTATTCGGGAATCCGGGGTCCCT36080.6109972735432084No Hit
ATATTATAGTAGTCCATTCACTTTCGGCCCTGGGACCACAGTGAGTGCCATACGAACTGTGGCTGCACCAT29400.49787471846370085No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28150.47670657567187685No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23950.40558161589134817No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19760.33462600125315406No Hit
GTTTTGCACAGCCCCAACAATAAGAACTACTTAGCCTGGTACCAGCAGAA19090.32327987671673636No Hit
CTATAATATTGCTGACAGTAATAAATTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC17810.3016036984979086No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16480.2790807945674078No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16070.2721376437316896No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15640.2648558026113021No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15080.25537247464056495No Hit
GCCCCAACAATAAGAACTACTTAGCCTGGTACCAGCAGAAACCACGACAGCCTCCTAAACTGCTCATTTACTGGA14790.25046146551286175No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13880.23505105756041386No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13720.23234153528306042No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13580.2299707032903761No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13390.22675314558601886No Hit
GTTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAGGCTAAGTAGTTCTTA12600.21337487934158608No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11870.20101268395116087No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11830.20033530338182248No Hit
CTGTGGTCCCAGGGCCGAAAGTGAATGGACTACTATAATATTGCTGACAGTAATAAATTGCCACATCTTCAGC11560.1957629845387885No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC11390.19288411711910045No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTACTGTCAGCAATATTATAGTAGT11310.1915293559804237No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11240.19034393998408156No Hit
GGACCACAGTGAGTGCCATACGAACTGTGGCTGCACCATCTGTCTTCATC11000.18627965656805134No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10440.1767963285973142No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10200.172732045181284No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9640.16324871721054682No Hit
GTGCAGCCACAGTTCGTATGGCACTCACTGTGGTCCCAGGGCCGAAAGTG9610.16274068178354303No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9560.1618939560718701No Hit
ACTATAATATTGCTGACAGTAATAAATTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT9230.15630556637482854No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGTCACCATCAACTGCAAGTCCAGCCGGAGTGTTTTGCA9100.15410407952447885No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9000.1524106281011329No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8690.14716092868876057No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC8650.14648354811942219No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8600.14563682240774925No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8530.14445140641140708No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGTCACCATCAACTGCAAGTCCAGCCGGAGTGTTTTGCACAGCCCCAA8470.14343533555739954No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8300.14055646813771147No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGTCACCATCAACTGCAAGTCCAGC8270.1400484327107077No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8270.1400484327107077No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC8180.13852432642969637No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7860.1331052818749894No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7360.12463802475825982No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7360.12463802475825982No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7270.1231139184772485No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7220.12226719276557552No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7200.12192850248090634No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7190.12175915733857175No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7180.12158981219623716No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7090.1200657059152258No Hit
CAGTGAGTGCCATACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG7090.1200657059152258No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7010.11871094477654909No Hit
ACTTAGCCTGGTACCAGCAGAAACCACGACAGCCTCCTAAACTGCTCATT6910.11701749335320316No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTACTGTCAGCAATATTATAGTAGTCCATTCACTTT6860.11617076764153021No Hit
GAACTACTTAGCCTGGTACCAGCAGAAACCACGACAGCCTCCTAAACTGC6860.11617076764153021No Hit
GTGCAAAACACTCCGGCTGGACTTGCAGTTGATGGTGACCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA6750.1143079710758497No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6700.11346124536417673No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6620.11210648422549999No Hit
ATAGTAGTCCATTCACTTTCGGCCCTGGGACCACAGTGAGTGCCATACGA6580.11142910365616163No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6540.11075172308682324No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6530.11058237794448866No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6520.11041303280215407No Hit
CAATAAGAACTACTTAGCCTGGTACCAGCAGAAACCACGACAGCCTCCTA6430.10888892652114275No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6390.10821154595180436No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT6360.1077035105248006No Hit
ATTTATTACTGTCAGCAATATTATAGTAGTCCATTCACTTTCGGCCCTGGGACCACAGTGAGTGCCATACGAA6310.10685678481312764No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT6280.10634874938612388No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC6110.1034698819664358No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6100.10330053682410119No Hit
GGCTGAAGATGTGGCAATTTATTACTGTCAGCAATATTATAGTAGTCCAT6060.10262315625476283No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC6030.10211512082775906No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6030.10211512082775906No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG5970.1010990499737515No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC5940.10059101454674774No Hit
GCCTGGTACCAGCAGAAACCACGACAGCCTCCTAAACTGCTCATTTACTGGACATCTATTCGGGAATCCGGGGTC5940.10059101454674774No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5930.10042166940441312No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG5920.10025232426207854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)