FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590510
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTACTGGACATCTATTCGGGAATCCGGGGTCCCT33070.5600243857004962No Hit
ATATTATAGTAGTCCATTCACTTTCGGCCCTGGGACCACAGTGAGTGCCATACGAACTGTGGCTGCACCAT29760.5039711435877462No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28340.4799241333762341No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19970.3381822492421805No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19600.33191647897580057No Hit
CTATAATATTGCTGACAGTAATAAATTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC19150.3242959475707439No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17560.2973700699395438No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16370.2772179980017273No Hit
GTTTTGCACAGCCCCAACAATAAGAACTACTTAGCCTGGTACCAGCAGAA16070.2721376437316896No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14980.253679023217219No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13490.22844659700936482No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13110.2220114816006503No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13030.22065672046197357No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12760.21608440161893955No Hit
GTTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAGGCTAAGTAGTTCTTA12260.20761714450220994No Hit
CTGTGGTCCCAGGGCCGAAAGTGAATGGACTACTATAATATTGCTGACAGTAATAAATTGCCACATCTTCAGC11810.19999661309715333No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11790.19965792281248412No Hit
GCCCCAACAATAAGAACTACTTAGCCTGGTACCAGCAGAAACCACGACAGCCTCCTAAACTGCTCATTTACTGGA11720.19847250681614198No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11610.19660971025046145No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11410.19322280740376963No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11290.19119066569575452No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11030.18678769199505513No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTACTGTCAGCAATATTATAGTAGT10890.1844168600023708No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10890.1844168600023708No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10860.18390882457536706No Hit
GGACCACAGTGAGTGCCATACGAACTGTGGCTGCACCATCTGTCTTCATC10830.18340078914836327No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10820.1832314440060287No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC10230.17324008060828774No Hit
GTGCAGCCACAGTTCGTATGGCACTCACTGTGGTCCCAGGGCCGAAAGTG9990.16917579719225753No Hit
ACTATAATATTGCTGACAGTAATAAATTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT9210.15596687609015936No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9150.1549508052361518No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8730.14783830925809893No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8580.14529813212308004No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8530.14445140641140708No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8470.14343533555739954No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8260.1398790875683731No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8200.13886301671436554No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC8150.13801629100269258No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGTCACCATCAACTGCAAGTCCAGCCGGAGTGTTTTGCACAGCCCCAA8090.13700022014868504No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGTCACCATCAACTGCAAGTCCAGCCGGAGTGTTTTGCA8000.13547611386767372No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7990.13530676872533912No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7710.13056510473997054No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7430.12582344075460195No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGAAT7370.1248073699005944No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC7350.12446867961592521No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7120.12057374134222959No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGTCACCATCAACTGCAAGTCCAGC7100.12023505105756042No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7020.11888028991888368No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTACTGTCAGCAATATTATAGTAGTCCATTCACTTT6950.11769487392254153No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG6820.11549338707219182No Hit
GGCTGAAGATGTGGCAATTTATTACTGTCAGCAATATTATAGTAGTCCAT6750.1143079710758497No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6720.11379993564884591No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC6710.11363059050651131No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG6650.11261451965250376No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6590.11159844879849622No Hit
ATAGTAGTCCATTCACTTTCGGCCCTGGGACCACAGTGAGTGCCATACGA6560.11109041337149243No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6550.11092106822915784No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6550.11092106822915784No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6530.11058237794448866No Hit
CAGTGAGTGCCATACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG6500.11007434251748488No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6480.10973565223281569No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6390.10821154595180436No Hit
GTGCCATACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTG6380.10804220080946977No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6370.1078728556671352No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6280.10634874938612388No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6250.10584071395912008No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATGGCACTCACTGTGGTCCCAGGGCCGAAAGTGAATGGACTA6240.10567136881678549No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6210.1051633333897817No Hit
GTATGGCACTCACTGTGGTCCCAGGGCCGAAAGTGAATGGACTACTATAATATTGCTGACAGTAATAAATTGCCA6190.10482464310511252No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG6030.10211512082775906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATCGG200.006340321653.73340664
AAGCGTG200.00707399452.26629648
AATACGC200.00774639951.0797355
GGTATCA7100.044.1476551
TGGTATC2050.039.877242
ACGATTA450.001323914739.5671769
CGATTGT350.001431663338.94428616
GTGGTAT2100.038.9376831
GTATCAA18850.038.860221
GGGATAC451.0754512E-437.86891618
TGTTTAG451.0808313E-437.83683810
CGCTACG1200.036.92532319
TTAACGG754.1532985E-836.65874535
GGTCTGG14050.036.521670
TACGGTC1250.035.45432322
GGACCCG400.002687105734.25628732
GGATACT501.9974438E-434.08491519
AACACGG400.002766623434.0531545
TAACCGT957.568815E-933.18575753
CACTGAT2553.6379788E-1233.0783970
Produced by FastQC (version 0.11.5)