FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897766
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT33090.3685815680255211No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC32120.35777697083649856No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28200.3141130316808612No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA27950.3113283416836904No Hit
CTCCTGCACTGGTACCAGCAGTGATGTTGGTCGTTTTAACCATGTCTCCTGGTACCAACAGCACCCAGGCAAAGT26000.2896077597057585No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC25390.28281311611266186No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC25010.27858038731696233No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTCGTCCCAGTTCCAAAGACATA24530.2732337825223945No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG24430.2721199065235262No Hit
GATTATTACTGCTGCTCATATGCAGACACCTATTATGTCTTTGGAACTGGGACGAAGGTCACCGTCCTGGGTCAG23230.25875339453710655No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21780.24260219255351614No Hit
GTGTCCGGGTCTCCTGGACAGTCACTCACCATCTCCTGCACTGGTACCAG21390.2382580761579298No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC20190.22489156417151018No Hit
CCTATTATGTCTTTGGAACTGGGACGAAGGTCACCGTCCTGGGTCAGCCC20120.22411185097230235No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC18910.2106339513859959No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18370.20461902099210708No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17820.1984927029983314No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG17770.19793576499889726No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16690.1859059042111196No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTCGTCCCAGTTCCAAAGACATAATAGGTGT16500.18378953981326981No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16150.1798909738172308No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15470.17231661702492632No Hit
GACTATCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCT15230.1696433146276424No Hit
GTCACTCACCATCTCCTGCACTGGTACCAGCAGTGATGTTGGTCGTTTTAACCATGTCTCCTGGTACCAACAGCA14920.16619029903115068No Hit
CTCCTGGACAGTCACTCACCATCTCCTGCACTGGTACCAGCAGTGATGTT14740.1641853222331877No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCACTCAC14640.1630714462343194No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG14600.16262589583477208No Hit
ACCCAGGACGGTGACCTTCGTCCCAGTTCCAAAGACATAATAGGTGTCTGCATATGAGCAGCAGTAATAATCAGC14390.16028675623714866No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCACTCACCATCTCCTG14150.15761345383986475No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13830.15404905064348615No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATAGTCAGGGAGG13450.1498163218477866No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12770.14224196505548217No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC12350.1375636858602353No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12300.13700674786080114No Hit
GGATGAGGCTGATTATTACTGCTGCTCATATGCAGACACCTATTATGTCTTTGGAACTGGGACGAAGGTCACCGT12240.13633842226148016No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12120.1350017710628382No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11530.1284299026695152No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11070.12330607307472104No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11070.12330607307472104No Hit
TAATAGGTGTCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGC10930.12174664667630541No Hit
CCCCTGAGGGCCGCTTAGCGACATCATAAACCATAAGTTTGGGGACTTTGCCTGGGTGCTGTTGGTACCAGGAGA10860.1209669334770976No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10590.11795946828015319No Hit
GACCCAGGACGGTGACCTTCGTCCCAGTTCCAAAGACATAATAGGTGTCTGCATATGAGCAGCAGTAATAATCAG10100.1125014758856985No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC9980.11116482468705655No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCACTCAC9950.11083066188739604No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC9900.11027372388796189No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT9740.10849152228977262No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA9720.10826874708999895No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9610.10704348349124382No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9490.10570683229260186No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGACAACACGG9460.10537266949294137No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC9390.10459295629373355No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG9380.10448156869384673No Hit
CACCTATTATGTCTTTGGAACTGGGACGAAGGTCACCGTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACT9370.10437018109395989No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG9210.10258797949577061No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCACTCACCATCTCCTGCA9190.10236520429599695No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCACTCACCATCT9150.10191965389644964No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9110.10147410349690231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)