EGAF00004692250 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00004692250
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	623930
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

$Per base sequence content$

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA	1864	0.2987514625038065	No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT	1403	0.2248649688266312	No Hit
ATCTATGATAGTGGGGGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT	1225	0.19633612745019474	No Hit
ATCATAGATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGAAGTAAGAACTA	1177	0.1886429567419422	No Hit
GTCTATTACTGTGCGAGGACGTTTTACTATGATAGTTGTGCTGATTGCCC	1167	0.1870402128443896	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1110	0.1779045726283397	No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC	959	0.15370313977529532	No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC	949	0.15210039587774268	No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC	938	0.15033737759043483	No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT	931	0.14921545686214802	No Hit
GTCTGGGACAGACTTCACTCTCACCATTAGCAGCCTACAGCCTGAAGATT	912	0.14617024345679805	No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG	888	0.14232365810267178	No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA	854	0.1368743288509929	No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT	835	0.13382911544564294	No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT	828	0.13270719471735612	No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA	773	0.12389210328081676	No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC	754	0.1208468898754668	No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT	696	0.11155097526966166	No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG	693	0.11107015210039586	No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTTTGGCCAGGGGACCAAGCTGGA	678	0.10866603625406697	No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG	654	0.1048194508999407	No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT	647	0.10369753017165388	No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT	643	0.10305643261263282	No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT	635	0.10177423749459073	No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAGTTGCTACGCTGCTGACAGTAATAAACTG	626	0.1003317679867934	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGAC	20	0.0077053164	51.14882	25
TACGGAT	600	0.0	48.12551	70
GGTATCA	825	0.0	46.221714	1
GGTACGG	30	6.733548E-4	45.396324	1
GTATCAA	2350	0.0	37.52441	1
CGGGTAT	40	0.002765832	34.05544	17
TATCAAC	2580	0.0	33.898964	2
ATCAACG	2630	0.0	33.381218	3
TCAACGC	2625	0.0	33.05591	4
CAACGCA	2660	0.0	32.748886	5
AACGCAG	2710	0.0	32.270226	6
CTACGGA	615	0.0	31.508997	69
CCCTTAA	45	0.004168531	31.319336	55
ACGCAGA	2810	0.0	31.242918	7
AGAGTAC	2790	0.0	31.100986	11
CGCAGAG	2805	0.0	30.93467	8
GCAGAGT	2910	0.0	29.818474	9
CAGAGTA	2975	0.0	29.166975	10
CGTCCAA	695	0.0	29.1097	68
AAGATCG	60	5.6144805E-4	28.576706	34

Summary