FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743197
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22670.30503352408580764No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18330.2466371634976998No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15460.20802021536685428No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTACTCAGAGCCTCCTG15250.2051945850158168No Hit
GGTCTACTCAGAGCCTCCTGCATCCTAATGGAAACCACTTTTTCGATTGGTACCTGCAGAAGCCAGGGCAGTCTC15060.20263806231725906No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14840.1996778781399817No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14090.18958634117199072No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA13610.1831277575124765No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12820.172498005239526No Hit
GAGTTTGCAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT12630.16994148254096827No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12060.16227191444529512No Hit
CTGCAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG11930.1605227147041767No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11270.1516421621723446No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA10690.1438380402504316No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10620.1428961634667524No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10520.14155062520435363No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10260.13805222572211676No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10010.13468838006611977No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGTTTG9940.1337465032824406No Hit
GGCCAAAAGTGTACGGAGTTTGCAGAGCTTGCATGCAGTAATAAACCCCA9930.13361194945620072No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG9870.13280462649876143No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9830.1322664111938019No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9520.12809524258036564No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATTTATTGGGGTTCTATTCGGGCC9400.12648059666548708No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9310.12526961222932817No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9300.12513505840308828No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8970.12069478213717225No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8890.11961835152725321No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTGCAAACT8880.11948379770101332No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8860.11921469004853356No Hit
GTTTATTACTGCATGCAAGCTCTGCAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA8670.11665816734997585No Hit
ACTCAGAGCCTCCTGCATCCTAATGGAAACCACTTTTTCGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG8660.11652361352373598No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT8600.11571629056629669No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8560.11517807526133717No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8440.11356342934645862No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG8340.11221789108405981No Hit
GTCAGGGACCCCGGAGGCCCGAATAGAACCCCAATAAATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA8310.11181422960534018No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8160.10979592221174196No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT8150.1096613683855021No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG7940.10683573803446463No Hit
TCCTAATGGAAACCACTTTTTCGATTGGTACCTGCAGAAGCCAGGGCAGT7920.10656663038198487No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7650.1029336770735081No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)