FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004692252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743197
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22610.3042262011283684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20360.2739515902243954No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16150.21730442937740602No Hit
GGTCTACTCAGAGCCTCCTGCATCCTAATGGAAACCACTTTTTCGATTGGTACCTGCAGAAGCCAGGGCAGTCTC15360.2066746771044555No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14500.19510304804782583No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14450.19443027891662643No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTACTCAGAGCCTCCTG13600.18299320368623662No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13470.1812440039451182No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13010.1750545279380837No Hit
GAGTTTGCAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT12900.17357443584944504No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA12860.17303622054448553No Hit
CTGCAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG11680.1571588690481797No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA10850.14599090147026966No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG10750.14464536320787086No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10610.14276160964051254No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10010.13468838006611977No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9890.13307373415124119No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9840.13240096502004178No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9760.13132453441012276No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9510.12796068875412575No Hit
GGCCAAAAGTGTACGGAGTTTGCAGAGCTTGCATGCAGTAATAAACCCCA9490.127691581101646No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9270.12473139692436863No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA9190.12365496631444961No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8990.120963889789652No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTGCAAACT8940.1202911206584526No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGTTTG8890.11961835152725321No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8830.11881102856981392No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8780.11813825943861453No Hit
ACTCAGAGCCTCCTGCATCCTAATGGAAACCACTTTTTCGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG8680.11679272117621572No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8630.11611995204501634No Hit
GTCAGGGACCCCGGAGGCCCGAATAGAACCCCAATAAATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA8410.11315976786773897No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATTTATTGGGGTTCTATTCGGGCC8330.11208333725781994No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG8300.11167967577910029No Hit
GTTTATTACTGCATGCAAGCTCTGCAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA8290.11154512195286043No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8180.11006502986422174No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT8010.10777761481814377No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7930.10670118420822473No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7890.10616296890326522No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7800.1049519844671063No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG7750.1042792153359069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)