FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556047
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT22400.4028436445120646No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19750.3551858026389855No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15600.2805518238566165No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA15250.2742573919111154No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14500.2607693234564704No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT13150.23649080023810937No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12610.22677939095076494No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAACAAGGACAGCACCTACAGCC12600.22659955003803636No Hit
GCCTAGAGCCTGAGGATTTTGCAGTTTATTATTGTCAACAACGTAACAACTGGCCTCGAACTTTTGGCCAGGGGA12360.22228336813254995No Hit
GTCCCAGACCCACTGCCAGTGAACCTGGTTGGGATGCCAGTGGCCCTCTT12330.22174384539436412No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG11580.20825577693971914No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10200.1834377309831723No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCAGGCT9990.17966107181587168No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGTTGTCCTGCTCT9900.17804250360131427No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9790.17606425356129968No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9720.17480536717219947No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC9680.1740860035212851No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT9330.16779157157578406No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC9260.16653268518668385No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9250.16635284427395525No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9180.16509395788485506No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTATTATTGTCAACAACGTAACAACTGG8820.15861968502662546No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8790.15808016228843963No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8540.15358413947022465No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA8420.15142604851748143No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8250.14836875300109523No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCAGTGAACCTGGTTGGGATGCCA7910.14225416196832283No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7540.1356000481973646No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATT7490.13470084363372162No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7420.1334419572446214No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7330.13182338903006402No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGTT7180.129125775339135No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7090.12750720712457758No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7010.1260684798227488No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT6910.12427007069546278No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6810.12247166156817679No Hit
GTTGTTACGTTGTTGACAATAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT6760.12157245700453378No Hit
GGCTGTAGGTGCTGTCCTTGTTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6640.1194143660517906No Hit
GTGTTAGCAGCTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCC6550.11779579783723318No Hit
CGTAACAACTGGCCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT6390.11491834323357558No Hit
GCAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATTCATCCAAGAGGGCCACTGGCATCCCAACCAGGTT6340.11401913866993257No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6320.11365945684447538No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6120.11006263858990338No Hit
ATAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGA6030.10844407037534597No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6020.10826422946261736No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5930.10664566124805996No Hit
CTGCTACTCTGGCTCCCAGATATCACCGGAGAGGTTGTGTTGACACAGTC5840.10502709303350256No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5770.10376820664440235No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT5650.10161011569165916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGG100.007736799140.8130670
ATTCCGT150.002253944869.7662944
TCGTATT309.14725E-657.47049740
CCTAACG301.0911561E-555.785212
TATTCCG252.4727563E-455.6149143
TCGTAAG252.5724736E-455.17167740
TCTAGAC252.9798952E-453.5538023
CGGCTTA200.00673182852.92506448
TAATCGC850.052.0336335
CTTATAC8750.051.267861
TCGTATG5550.049.70421240
TGCCGTC4800.049.5338845
TTATACA9250.048.8497542
TGTACGC250.00956064948.42930668
GCTAATC7150.048.32278433
TATACAC9300.048.227213
GTCGTAT507.144081E-848.1191539
CCGTCTT5050.048.0585947
CTAACGC352.7165612E-547.815893
CGTATGC5800.047.6810941
Produced by FastQC (version 0.11.5)