FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556047
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77111.3867532780502367No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19860.35716405267900014No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA14360.25825155067827No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14100.2535756869473264No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13810.248360300478197No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAACAAGGACAGCACCTACAGCC13040.23451255019809478No Hit
GCCTAGAGCCTGAGGATTTTGCAGTTTATTATTGTCAACAACGTAACAACTGGCCTCGAACTTTTGGCCAGGGGA12520.22516082273620752No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT12380.22264304995800716No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11410.20519848142333294No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG11300.20322023138331832No Hit
GTCCCAGACCCACTGCCAGTGAACCTGGTTGGGATGCCAGTGGCCCTCTT10810.1944080266596169No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA9760.17552473082311387No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC9740.17516504899765667No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT9670.17390616260855649No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGTTGTCCTGCTCT9630.17318679895764208No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCAGGCT9470.17030934435398448No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9320.16761173066305546No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8820.15861968502662546No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8730.15700111681206805No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8520.15322445764476744No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8470.15232525308112443No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTATTATTGTCAACAACGTAACAACTGG8470.15232525308112443No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8070.14513161657198043No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8050.14477193474652322No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC7970.14333320744469444No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCAGTGAACCTGGTTGGGATGCCA7970.14333320744469444No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7930.14261384379378No Hit
GGCTGTAGGTGCTGTCCTTGTTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7650.13757829823737922No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7640.1373984573246506No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7340.13200322994279262No Hit
GTTGTTACGTTGTTGACAATAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT7320.13164354811733542No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT7260.1305645026409638No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7160.1287660935136778No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7110.12786688895003478No Hit
GTGTTAGCAGCTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCC7020.1262483207354774No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6710.12067325244089079No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATT6520.11725627509904739No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6440.11581754779721858No Hit
GCAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATTCATCCAAGAGGGCCACTGGCATCCCAACCAGGTT6320.11365945684447538No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGTT6310.11347961593174678No Hit
GAGCAGGACAACAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6210.11168120680446077No Hit
GTGTATTACTGTGCGAGAGGGACCTGGGACTATGAGTCGGGGAGTTCTTCCTCCGACGACTCTGGCCCCTGGGGC6200.11150136589173218No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6120.11006263858990338No Hit
CTGCTACTCTGGCTCCCAGATATCACCGGAGAGGTTGTGTTGACACAGTC5950.10700534307351718No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCAGTGAACCTGGTTGGGATGCCAGTGG5730.10304884299348796No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5730.10304884299348796No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5680.10214963842984497No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5640.10143027477893056No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA5630.10125043386620197No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5600.10071091112801615No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAT150.002676921766.8051115
CGGCGTA200.00680154152.78737347
CCCGTAT200.00832427250.1579918
TGGTATC1900.042.19652
GTGGTAT1950.041.1256371
ATAGCGG752.158049E-839.48400563
CGAACCT654.0501072E-738.58257353
GTATCAA12000.038.148311
AGCGGCT803.3702236E-837.53650365
GGTATCA4650.037.366841
TAGCGGC803.5990524E-837.25772564
ATTGGGT400.00302743833.43263617
TCTATAT400.003039484133.405562
AATAGCG901.1119664E-732.77262562
TCAACGC13250.032.772324
ATCAACG13450.032.2820933
TATCAAC14000.032.2125052
CAACGCA13500.032.1654245
AATCGTT751.7080583E-632.09995739
CCGAGTA450.00406979931.47234749
Produced by FastQC (version 0.11.5)