FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880447
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG56480.6414923328718254No Hit
GATTATTACTGTGCAACATGGGATGACAGCCTGAATGCTTGGGTGTTCGG53600.6087816756715623No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC52380.5949250778297842No Hit
GCCTTGGGCTGACCGAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCA42580.4836179804122224No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35940.4082017429782826No Hit
GTATTATAATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTA29520.33528423630269627No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29200.33164971883600036No Hit
ATTATAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG28540.32415352656094004No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGAGACCC25450.2890577172731578No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCACCTTGGTCCCTC25440.2889441386023236No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG24070.27338386069803183No Hit
GTGCAGGGAGAAGGGCTTGATGCCATGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23620.26827282051049073No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21360.24260404090195092No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCATGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21340.24237688356028245No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20020.2273844990101619No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCATGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19830.2252265042643112No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT19250.21863894135592488No Hit
GAGAAGGGCTTGATGCCATGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19130.2172759973059139No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18090.20546381553915227No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17790.20205645541412487No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17730.20137498338911938No Hit
GTATTACTTCCGATATTGGACCTGCTTCCAGAACAAGAGATGGTGACCCT17410.1977404659224235No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17280.1962639432015783No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16910.19206153238071116No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16810.1909257456723687No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16200.18399744675147964No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15970.18138513732229197No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15920.18081724396812074No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15880.18036292928478376No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGAGACCCCCGGGCAGAGGGTCAC15840.17990861460144678No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15530.17638767580558512No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC15500.17604693979308236No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG14550.16525696606382895No Hit
CCCCTGAGGGCCGCTGAGTATTATAATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTA14540.1651433873929947No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAACATGGGATGACAGCCTGAATGCTTGGGTGTTCGGCGGAGGGAC13780.15651140840959196No Hit
TTATAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC13630.15480772834707823No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13320.1512867895512166No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA12690.14413133328865904No Hit
GAGTATTATAATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG12620.14333628259281933No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC12420.1410647091761344No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12250.13913387177195222No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCACCTTGGTC12150.13799808506360975No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT12100.13743019170943852No Hit
CCTTGGTCCCTCCGCCGAACACCCAAGCATTCAGGCTGTCATCCCATGTTGCACAGTAATAATCAGCCTCATCCT11970.1359536689885933No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11640.13220557285106316No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11500.13061547145938368No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11490.13050189278854946No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA11430.12982042076354397No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11340.12879821272603575No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAG11330.1286846340552015No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCATGGG11300.12834389804269877No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11230.12754884734685903No Hit
ACATGGGATGACAGCCTGAATGCTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTCGGTCAGCCCAAGG10990.12482295924683712No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT10960.12448222323433439No Hit
GAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAA10960.12448222323433439No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10950.12436864456350012No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT10680.12130202045097546No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10530.11959834038846176No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAA10420.11834897500928505No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10250.11641813760510286No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC10210.11596382292176587No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC10090.11460087887175492No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10080.11448730020092067No Hit
CCCAAGCATTCAGGCTGTCATCCCATGTTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGG10030.11391940684674944No Hit
GTGCAACATGGGATGACAGCCTGAATGCTTGGGTGTTCGGCGGAGGGACC10020.1138058281759152No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9970.11323793482174395No Hit
GACCGAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAGCATTCAGG9960.11312435615090971No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG9660.10971699602588231No Hit
GGACCAAGGTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG9640.10948983868421382No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGTAGATACA9490.10778615862170011No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT9450.10733184393836312No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9340.1060824785591864No Hit
ATGTTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATG9230.10483311318000971No Hit
ATAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG9150.10392448381333573No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT9070.10301585444666177No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9050.10278869710499325No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8890.10097143837164531No Hit
GATTATTACTGTGCAACATGGGATGACAGCCTGAATGCTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCT8860.10063070235914258No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8850.10051712368830833No Hit
TCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8840.10040354501747407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG301.0038695E-556.580522
CTAACGC301.0038695E-556.580523
GGTATCA10600.049.0008621
CGCGTAT306.398177E-445.87151739
ATCGCGT306.500461E-445.7241237
CGTATGC1250.044.20715341
GTGGTAT1800.043.3783951
TTGCCGA350.001119396240.9447360
GCCAGAG20300.039.82244570
GCGTATG350.001357038439.3728440
CGTGAAA556.044884E-638.7560357
ACGAGAC2750.038.2668322
TCTGGAG3950.036.73332670
CACGAGA3000.035.07593521
ATGCCGT1500.034.70328544
TGGTATC2250.034.7027172
TCCGAGC3050.034.49896213
TCGTATG1300.034.45123340
GTATCAA25100.034.354071
CGAGACA3100.033.9463823
Produced by FastQC (version 0.11.5)