FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880447
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAACATGGGATGACAGCCTGAATGCTTGGGTGTTCGG53110.6032163208006842No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51130.5807277439755033No Hit
ATTATAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG37020.42046823942838124No Hit
GCCTTGGGCTGACCGAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCA33340.3786712885613785No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33090.3758318217905223No Hit
GTATTATAATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTA30300.3441433726277675No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30220.3432347432610935No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGAGACCC29740.33778296706104965No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG26790.3042772591649469No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCATGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26270.29837116828156607No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25710.2920107627148482No Hit
GTGCAGGGAGAAGGGCTTGATGCCATGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23640.2684999778521592No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG23430.26611482576464No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT22750.25839147614791125No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21690.24635213703948108No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCATGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21610.2454435076728071No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCACCTTGGTCCCTC20740.23556216331022764No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGAGACCCCCGGGCAGAGGGTCAC20470.232495539197703No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20200.2294289150851783No Hit
TTATAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC17910.2034193994641358No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17890.20319224212246734No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17630.20023919668077694No Hit
GTATTACTTCCGATATTGGACCTGCTTCCAGAACAAGAGATGGTGACCCT17360.19717257256825224No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC17220.1955824711765728No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16750.1902442736473632No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16460.18695049219317006No Hit
GAGAAGGGCTTGATGCCATGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16210.1841110254223139No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15810.17956787858894402No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15790.17934072124727551No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG15650.1777506198555961No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15150.17207168631388375No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14900.16923221954302758No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14870.16889148353052483No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14790.16798285416385086No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14330.1627582353054755No Hit
CCCCTGAGGGCCGCTGAGTATTATAATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTA14120.16037308321795635No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAACATGGGATGACAGCCTGAATGCTTGGGTGTTCGGCGGAGGGAC13830.15707930176376317No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13750.1561706723970892No Hit
GAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAA13630.15480772834707823No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13230.1502645815137084No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA12390.14072397316363167No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT12340.1401560798094604No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12180.13833882107611248No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA11810.13413641025524536No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT11680.13265988753440014No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11580.13152410082605767No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11580.13152410082605767No Hit
ATAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG11580.13152410082605767No Hit
GAGTATTATAATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG11570.13141052215522342No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11310.12845747671353303No Hit
CTCTTGTTCTGGAAGCAGGTCCAATATCGGAAGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCC11250.12777600468852754No Hit
ACATGGGATGACAGCCTGAATGCTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTCGGTCAGCCCAAGG11130.12641306063851657No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAA10910.12391432988016315No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10800.12266496450098643No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10660.12107486310930697No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC10640.12084770576763848No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG10460.11880328969262205No Hit
GATTATTACTGTGCAACATGGGATGACAGCCTGAATGCTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCT10340.11744034564261108No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10330.11732676697177684No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT10230.11619098026343438No Hit
GTGCAACATGGGATGACAGCCTGAATGCTTGGGTGTTCGGCGGAGGGACC10120.11494161488425766No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT10050.11414656418841793No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10010.11369224950508094No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAG9990.11346509216341245No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCATGGG9970.11323793482174395No Hit
GGACCAAGGTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG9800.11130709741756176No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA9510.1080133159633686No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGTAGATACA9460.10744542260919737No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9400.10676395058419189No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC9360.10630963590085492No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGAGACCCCCGGGCAGAGG9340.1060824785591864No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9290.10551458520501518No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9270.10528742786334669No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC9260.10517384919251244No Hit
CCCTCAGCGTCTGAGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG9190.10437879849667271No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9160.10403806248416997No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAGTATTACTT9110.10347016912999873No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCACCTTGGTC8950.1016529103966508No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8840.10040354501747407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAACG306.8756536E-445.206363
GGTATCA11050.042.6664051
GTACGGA405.5288823E-542.3978231
TCTGGAG4200.040.51817770
CATGTTG15400.036.834770
GTGGTAT3350.036.449471
GTATCAA27550.036.3190081
TGGTATC3500.035.8421862
TACGGAG400.002827240233.9047742
TATCAAC30700.032.4690672
CCGATAT15550.031.4634770
GCCAGAG12900.031.33091470
ATCAACG32400.030.765443
TCAACGC32650.030.4329434
CCGTGTC3700.030.24626212
TTGAACG450.00502576830.14613510
CTCGCTA450.00502576830.14613510
CTCTGGA4450.030.11751469
CAACGCA33050.030.0663225
AACGCAG33500.029.7636876
Produced by FastQC (version 0.11.5)