FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322839
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACTATCATACTGCTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGT47600.35983214888584325No Hit
ATAGTACACCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA46950.3549184745838307No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37850.2861270343556548No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34300.25929081316774No Hit
GTATGATAGTACACCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT34180.2583836732965992No Hit
CTATCATACTGCTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTC28140.2127242997825132No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27990.21159037494358723No Hit
GTAATAGACGGCCGTGTCCTCAGATCTCAGGCTGTTAAACTCCATGTAGG27250.2059963457382191No Hit
CTCCTGTAGGGCCAGTCAGAGTATTAGAAGTACCTATTTAGCCTGGTACC26580.20093148145768308No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTGGCTTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA26110.19737851696238168No Hit
CTATTACTGTCAGCAGTATGATAGTACACCGTTCACTTTTGGCCAGGGGA24690.18664402848721576No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23380.1767410848939289No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22630.17107146069929902No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21610.16336077179460237No Hit
GTATTAGAAGTACCTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCGT21220.1604125672133948No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATT20930.1582203125248046No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAATAGGTACTTCTAATACTCTGA20160.15239949835165126No Hit
GTCTATTACTGTGCGACAGATGGGACATATACTATGGGTCCCGTCTGGGGCCAGGGAAGCCTGGTCACCGTCGC20110.1520215234053426No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19830.1499048637060141No Hit
CAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19370.14642749419997445No Hit
GTCTATTACTGTCAGCAGTATGATAGTACACCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA19280.14574713929661887No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19210.14521797437178674No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19020.14378166957581384No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18540.14015311009125073No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGAAGCCAGTGGCCCTGCT17910.1353906257677616No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17820.13471027086440604No Hit
GTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17680.1336519410147418No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT17620.13319837107917137No Hit
GAGTATTAGAAGTACCTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGG17430.13176206628319848No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17330.13100611639058116No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17120.1294186216160848No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17000.128511481744944No Hit
GTATATGTCCCATCTGTCGCACAGTAATAGACGGCCGTGTCCTCAGATCTCAGGCTGTTAAACTCCATGTAGG16910.12783112684158845No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16910.12783112684158845No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15830.11966686800132141No Hit
TCTATGGTGCGTCCAGCAGGGCCACTGGCTTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT15760.11913770307648927No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTATTAGAAGTA15700.11868413314091888No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15470.11694544838789905No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15460.11686985339863733No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15020.11354367387112112No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14590.11029308933286668No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13920.10522822505233063No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13880.10492584509528372No Hit
GACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATTTTGCAGTCTATTACTGTCAGCAGTATGATAGTACA13760.10401870522414292No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13510.10212883049259963No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13490.10197764051407616No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13350.10091931066441191No Hit
TCCCTATGGTTCCTGTGGCAATCTACGCTCAGAATTTCCAGGGCAGAGTC13300.10054133571810327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAA150.00248569968.07098420
TCGTATC200.002768888466.2422669
CAACGTA200.006798573352.79752754
CGCGTAT352.3063152E-549.16479539
CGCGGAA9600.046.25388770
ATCGCGT451.929533E-645.76120837
ACTACGC4250.045.69251327
GGTATCA11750.043.179491
GCGTATG405.037779E-543.07352440
GAGACTA4700.042.01236324
TGCATCG2050.041.74497234
CGAGACT4750.041.5701323
ACGAGAC4800.041.1324422
ATCTCCG4850.040.70070310
CTTATAC4700.040.571331
CGTATGC602.9155308E-740.24241
TACGCTG4900.039.67479329
TCTCCGA5000.039.47968311
CACGAGA5100.039.37737321
TCGATAC350.00142609438.97874529
Produced by FastQC (version 0.11.5)