FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322839
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACTATCATACTGCTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGT49250.372305322114029No Hit
ATAGTACACCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA41080.3105442158871941No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39170.2961055729382034No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37400.28272525983887686No Hit
GTATGATAGTACACCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT33620.2541503538979422No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31640.23918254602411934No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27830.21038085511539953No Hit
CTCCTGTAGGGCCAGTCAGAGTATTAGAAGTACCTATTTAGCCTGGTACC27330.20660110565231293No Hit
CTATCATACTGCTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTC26550.20070469648989786No Hit
GTAATAGACGGCCGTGTCCTCAGATCTCAGGCTGTTAAACTCCATGTAGG25000.18898747315432946No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTGGCTTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA24090.18210832913151184No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22290.16850123106440013No Hit
CTATTACTGTCAGCAGTATGATAGTACACCGTTCACTTTTGGCCAGGGGA22230.16804766112882974No Hit
GTATTAGAAGTACCTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCGT21790.16472148160131356No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21330.16124411209527387No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATT21240.1605637571919183No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21100.15950542734225406No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20080.15179473843755742No Hit
CAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19040.1439328595543373No Hit
GTCTATTACTGTGCGACAGATGGGACATATACTATGGGTCCCGTCTGGGGCCAGGGAAGCCTGGTCACCGTCGC18660.1410602499623915No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAATAGGTACTTCTAATACTCTGA18390.13901918525232473No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18130.1370537155315197No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18030.1362977656389024No Hit
GTCTATTACTGTCAGCAGTATGATAGTACACCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA17500.1322912312080306No Hit
GTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17420.13168647129393676No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGAAGCCAGTGGCCCTGCT16880.12760434187380323No Hit
GTATATGTCCCATCTGTCGCACAGTAATAGACGGCCGTGTCCTCAGATCTCAGGCTGTTAAACTCCATGTAGG16820.12715077193823285No Hit
GAGTATTAGAAGTACCTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGG16780.12684839198118592No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT16320.12337102247514625No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16090.12163233772212642No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15880.12004484294763007No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15740.11898651309796582No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTATTAGAAGTA15560.11762580329125465No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15000.11339248389259765No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGA14850.11225855905367169No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14730.1113514191825309No Hit
TCTATGGTGCGTCCAGCAGGGCCACTGGCTTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT14660.11082225425769877No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14660.11082225425769877No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14560.11006630436508147No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14510.10968832941877281No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14500.10961273442951108No Hit
GACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATTTTGCAGTCTATTACTGTCAGCAGTATGATAGTACA14450.10923475948320241No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14440.10915916449394068No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14180.10719369477313566No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14050.10621095991273313No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14000.10583298496642447No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13620.10296037537447869No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13460.10175085554629097No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT502.0944409E-457.51709770
GCGGTAT200.005453594555.8229567
TAGACGC200.00782202850.957864
CGAGTAC200.00782319850.95593311
GGTATCA11050.043.3668021
CGCGGAA10800.043.27096670
GTATCAA28550.037.140611
CGTTATA400.002770482634.0465727
CCACGGG9400.031.35905670
TATCAAC34500.030.4304642
ATCAACG34700.029.9613343
TCAACGC34850.029.731514
CAACGCA35300.029.256265
AACGCAG36100.028.9843436
GGTACGT855.9808604E-527.72215768
ACGCAGA38200.027.3909637
GTACTGA1005.721995E-727.1888331
CGCAGAG38800.027.1425048
AGAGTAC39850.026.6820711
TGGTACG858.627071E-526.26962567
Produced by FastQC (version 0.11.5)