FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559051
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53100.3405918087349291No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50570.32436398809275646No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35440.22731777215754967No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGT34710.222635436557239No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32600.20910156242483408No Hit
GATCTATTCTGCGTCCAGTTTACCAACTGGGGTCCCATCAAGGTTCAGTGGTAGTGGATCTGGGACAGATTTCA32450.20813943867134557No Hit
GAATAGATCACGACCCTAGGGGCTTTCCCTGGTGTCTGCTGATACCAATT28290.18145653990793117No Hit
GTCGGAGACAGAGTCACTATCACTTGCCGGGCAAGTCAGAACATTATCACACATTTAAATTGGTATCAGCAGACA27500.17638935480622506No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27470.1761969300555274No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26680.17112974495382127No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25770.16529286084932435No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25560.16394588759444045No Hit
CTGCTACTCTGGCTCCGAGTCTCAGGTGCCAGATGTGACATCCAGATGAC25270.16208578167102936No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAGTTG24950.16003325099692056No Hit
ACTTACTACTGTCAACAGAGTCACAGTATCCCTGGCAACTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA24160.15496606589521447No Hit
GTCTACTACTGTGCGAGATCGGCATTTTGGAGTGTTTATTTTGGAGAAAATCACTACTTCTACGTTATGGACGT24140.15483778272808266No Hit
GTAGTAAGTTGCGATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCA23310.14951403129211296No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22940.14714079270017466No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22890.14682008478234518No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCT22770.14605038577955434No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22640.14521654519319765No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22520.14444684619040685No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22110.14181704126420494No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21210.13604429874327395No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21040.13495389182265363No Hit
GAGTCACAGTATCCCTGGCAACTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT20850.13373520173490155No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20850.13373520173490155No Hit
GTCTGCATCTGTCGGAGACAGAGTCACTATCACTTGCCGGGCAAGTCAGA20790.13335035223350614No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20770.13322206906637435No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAGTTGCCAGGGATA20410.13091297205800195No Hit
CAGTATCCCTGGCAACTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT20400.13084883047443607No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20340.13046398097304066No Hit
GGGATACTGTGACTCTGTTGACAGTAGTAAGTTGCGATATCTTCAGGTTG19540.12533265428776866No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18580.11917506226544225No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18160.11648111575567445No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18130.11628869100497674No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATATCGCAACTTACTACTGTCAACAGAGTCACAGTATC17770.11397959399660434No Hit
CCCTAGGGGCTTTCCCTGGTGTCTGCTGATACCAATTTAAATGTGTGATA17760.11391545241303844No Hit
CTGTGACTCTGTTGACAGTAGTAAGTTGCGATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC17210.11038766531691394No Hit
GTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATATCGCAACTTACTA16980.10891240889489824No Hit
GAGTGAAATCTGTCCCAGATCCACTACCACTGAACCTTGATGGGACCCCA16670.10692401980435536No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16560.10621846238513044No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16490.10576947130016914No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16390.10512805546451015No Hit
GTAGTAGACAGCCGAGTCTTCAGATGTCAGGCTGCTCAATTCCATGTAGG16290.10448663962885113No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16280.10442249804528525No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16260.10429421487815345No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16030.10281895845613775No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15930.10217754262047873No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15610.10012501194636993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC2350.053.6166227
CGATAGG200.006773303352.8477254
GACCGAT2650.047.5269726
TATCGCG306.492965E-445.7370736
ACGAGAC2950.043.81524722
CGTATGC651.1301381E-842.49795541
CCGATCA3050.041.3324128
TATCTCG1100.040.53967736
AGACCGA3200.040.41422325
GTATCAA42850.039.608241
GTATCGC350.001394661139.15632635
TATACAC3650.038.198233
AGTATCG1001.0913936E-1137.64538634
GAGACCG3450.037.47362524
CCCACGA3750.037.17960719
CGAGACC3450.036.482823
ATCTCCG3450.036.46758710
CGATCAG3500.036.0356129
CACGAGA3600.035.9030121
TATCAAC47750.035.2520262
Produced by FastQC (version 0.11.5)