FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559051
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54760.35123931160686855No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43250.27741234892251765No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGT34870.22366170189429338No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34770.22302028605863441No Hit
GATCTATTCTGCGTCCAGTTTACCAACTGGGGTCCCATCAAGGTTCAGTGGTAGTGGATCTGGGACAGATTTCA32270.20698489016715937No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31870.2044192268245234No Hit
GTCGGAGACAGAGTCACTATCACTTGCCGGGCAAGTCAGAACATTATCACACATTTAAATTGGTATCAGCAGACA29690.19043636160715716No Hit
GAATAGATCACGACCCTAGGGGCTTTCCCTGGTGTCTGCTGATACCAATT29290.18787069826452116No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28890.18530503492188516No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28690.18402220325056717No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26320.16882064794544888No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25470.16336861334234737No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAGTTG25110.16105951633397494No Hit
GTCTACTACTGTGCGAGATCGGCATTTTGGAGTGTTTATTTTGGAGAAAATCACTACTTCTACGTTATGGACGT24680.15830142824064128No Hit
GTAGTAAGTTGCGATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCA23390.15002716396064014No Hit
ACTTACTACTGTCAACAGAGTCACAGTATCCCTGGCAACTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA23000.14752564220157005No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22590.14489583727536817No Hit
CTGCTACTCTGGCTCCGAGTCTCAGGTGCCAGATGTGACATCCAGATGAC22580.14483169569180226No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22520.14444684619040685No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22400.14367714718761607No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22280.14290744818482526No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21600.13854582050234404No Hit
GGGATACTGTGACTCTGTTGACAGTAGTAAGTTGCGATATCTTCAGGTTG21530.13809682941738274No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21520.13803268783381686No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21030.13488975023908775No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20840.13367106015133565No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCT20820.13354277698420386No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20670.13258065323071536No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAGTTGCCAGGGATA20100.12892458296745907No Hit
GAGTCACAGTATCCCTGGCAACTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT20060.12866801663319544No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19920.12777003446327284No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19820.12712861862761385No Hit
GTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATATCGCAACTTACTA19690.12629477804125716No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19110.12257456619443495No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19030.12206143352590776No Hit
CAGTATCCCTGGCAACTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT18880.12109930977241926No Hit
GTCTGCATCTGTCGGAGACAGAGTCACTATCACTTGCCGGGCAAGTCAGA18780.12045789393676026No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17840.11442858508156566No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATATCGCAACTTACTACTGTCAACAGAGTCACAGTATC17300.11096493956900705No Hit
CTGTGACTCTGTTGACAGTAGTAAGTTGCGATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC16970.10884826731133233No Hit
CCCTAGGGGCTTTCCCTGGTGTCTGCTGATACCAATTTAAATGTGTGATA16960.10878412572776644No Hit
GAGTGAAATCTGTCCCAGATCCACTACCACTGAACCTTGATGGGACCCCA16860.10814270989210745No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16700.10711644455505304No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16220.10403764854388985No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16070.10307552479040134No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16030.10281895845613775No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15900.10198511786978104No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15770.10115127728342434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)