FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392514
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA63430.45550709005439083No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT60710.43597407279208683No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAGCAGC60080.4314498812938326No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG53310.38283277582846564No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCACTCAACC51740.3715582033645622No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC51420.3692602013337029No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCACTCAAC48990.35180974841186513No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46770.3358673593227788No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC42860.3077886470082168No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC42730.3068550836831802No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC40770.292779821244167No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCACTCAACCTGCTGTCATAGGA40760.2927080086807027No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC40570.29134356997488No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCCGGAGCCCAG35470.25471916260805993No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG34440.24732246857123158No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAGCAGCCCCCAAAGTCCTCAT34240.24588621730194457No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAGCAGCCCCCAAAGTCCTCATCTATGGTAACAGCAATCGG31300.22477332364342476No Hit
GATTATTACTGCCAGTCCTATGACAGCAGGTTGAGTGGTTATGTCTTCGG31170.22383976031838818No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29030.20847187173701665No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG28380.20380405511183372No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG28200.20251142896947535No Hit
GTCCTATGACAGCAGGTTGAGTGGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGC27850.19999798924822298No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27760.19935167617704383No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT27750.19927986361357947No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC27630.19841811285200725No Hit
TCACTGGGCTCCGGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT26550.1906623559978571No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA26500.19030329318053535No Hit
GGCCATCACTGGGCTCCGGGCTGAGGATGAGGCTGATTATTACTGCCAGT25880.18585091424574546No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG25440.18269116145331393No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGAGTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTG24490.17586896792420043No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG23960.1720629020605897No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC23750.1705548382278383No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGGTTGAGTGGTTATGTCTTCGGAACTGGGACCAAGGT22630.16251183111983075No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT22420.16100376728707932No Hit
GTAATAATCAGCCTCATCCTCAGCCCGGAGCCCAGTGATGGCCAGGGAGG21740.15612051297150334No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA20370.14628219177688698No Hit
GTGCAGGAGATGGTGACCATCTGCCCTGGGGCCCCAGACACTGAGGGCGG19590.14068081182666745No Hit
GTGTCTGGGGCCCCAGGGCAGATGGTCACCATCTCCTGCACTGGGAGCAG19500.14003449875548826No Hit
GCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGAGTTGGAGCCAGAGAAT19480.13989087362855956No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA19300.13859824748620123No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC19060.13687474596305674No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAACTCTGGCACCTCAG18660.13400224342448264No Hit
GCCTCATCCTCAGCCCGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC18290.13134517857630157No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC18200.13069886550512239No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG18020.12940623936276405No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT17090.12272767096057921No Hit
GTATATTACTGTGCGAAATTGTGGGGGCACCCCGTCTCCAGTTACCACTT16870.12114779456436345No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16760.12035785636625557No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16690.1198551684220051No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16640.11949610560468332No Hit
GGGCAGATGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG16630.11942429304121896No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGTTGGCTTTTT16410.11784441664500322No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT16390.11770079151807451No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT16390.11770079151807451No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16120.11576185230453698No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC16050.11525916436028652No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15860.11389472565446379No Hit
GTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGA15720.11288934976596285No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15480.11116584824281839No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC15450.11095041055242533No Hit
GTTATGATGTACACTGGTACCAGCAGCTTCCAGGAGCAGCCCCCAAAGTCCTCATCTATGGTAACAGCAATCGGC14530.10434365471370485No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGATGGTCACCATCTCCTG14500.10412821702331179No Hit
CATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAACTCTGGCACCTCAGCCTC14380.10326646626173956No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA14330.1029074034444178No Hit
CTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCGGGCTGAGGATG14260.10240471550016733No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14230.10218927780977427No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG13930.10003490090584367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT1550.050.9991937
TATCTCG1750.049.05599636
ATAACAC306.6734053E-445.483013
GGTATCA12700.044.3174861
GCCTATC2100.044.05005633
GAGCCTA2500.043.79582631
CGCATAA658.766292E-443.02905770
TCGTATG1900.041.6921140
CGTATGC1750.041.37908641
CCTATCT2200.040.51676634
GTATCAA33850.040.308461
CCCACGA2650.039.8977419
AGCCTAT2500.039.72001632
TCTCCGA2650.038.6065611
AGACCGG2850.037.11789325
ATCTCCG2800.036.5396610
ACGAGAC2900.036.46482522
GTGGTAT2900.036.4635161
GAGACCG3000.036.3864124
TATCAAC38650.035.303762
Produced by FastQC (version 0.11.5)