FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605545
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT23210.3832910848904706No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21640.35736402744635No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA20920.3454739119305749No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG18480.3051796315715595No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC18320.30253738367916505No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAACACTTTTGTCTTCGGAACTGGGACCAAGGTCACCGTCCTGGGT16180.26719731811838926No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC15640.2582797314815579No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC15090.249197004351452No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC14790.24424278955321238No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC14390.23763716982222624No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAAGTGTTGCTGCTTGTATATGAGCTG13410.22145340148131024No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT13110.21649918668307064No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT12830.21187525287138034No Hit
GTACAACCGTGTCTCCTGGTACCAGCAGCCCCCCGGCACAGCCCCCAAAG11990.19800345143630946No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11540.19057212923895003No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11170.18446193098778785No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAAGTGTTG10930.18049855914919619No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG10600.1750489228711326No Hit
ACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAAGTGTTGCTGC10490.17323237744511144No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAAGCAGCAGTGACGTT10070.166296476727576No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCAC9620.15886515453021657No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG9560.15787431157056867No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9230.1524246752925051No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG8970.1481310224673641No Hit
ATGTACAACCGTGTCTCCTGGTACCAGCAGCCCCCCGGCACAGCCCCCAAAGTCATGATTTATGAGGACTATAAT8960.14796588197408944No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG8790.14515849358842037No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG8630.1425162456960259No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC8400.13871801435070885No Hit
CTCCTGCACTGGAAGCAGCAGTGACGTTGGTATGTACAACCGTGTCTCCTGGTACCAGCAGCCCCCCGGCACAGC8380.13838773336415955No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG8370.13822259287088492No Hit
GTCAGTCACCATCTCCTGCACTGGAAGCAGCAGTGACGTTGGTATGTACAACCGTGTCTCCTGGTACCAGCAGCC8010.1322775351129974No Hit
GACCCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAAGTGTTGCTGCTTGTATATGAGCTGCAGTAATAAT7940.1311215516600748No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7910.13062613018025085No Hit
GTTGTACATACCAACGTCACTGCTGCTTCCAGTGCAGGAGATGGTGACTG7850.12963528722060294No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC7850.12963528722060294No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA7620.1258370558752859No Hit
GAATATCTCCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGT7350.12137826255687026No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA7350.12137826255687026No Hit
CGGTTGTACATACCAACGTCACTGCTGCTTCCAGTGCAGGAGATGGTGAC7310.12071770058377164No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA7200.11890115515775046No Hit
GCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA7160.11824059318465184No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG7070.11675432874517996No Hit
CCCCGGCACAGCCCCCAAAGTCATGATTTATGAGGACTATAATCGGCCCT7020.11592862627880669No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT6770.11180011394694037No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC6610.1091578660545459No Hit
GACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT6510.10750646112179937No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGT6450.10651561816215145No Hit
GCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCAC6360.10502935372267957No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCCAGAGAAG6340.10469907273613027No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC6220.10271738681683443No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6190.10222196533701046No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG6180.10205682484373581No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA6160.1017265438571865No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC6100.10073570089753857No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6080.10040541991098928No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGT250.001053970584.4839770
GCGATTT250.001053970584.4839770
TCGATTG250.006271631553.87998669
GGTATCA5750.053.7360651
GTACGAA200.007307457351.83904343
TACGAAT200.007513678651.4754738
TTATACG200.007608378351.31266835
ATTCCGT200.00777750851.0281229
CGTATGC1600.038.77495241
GACCGTA1700.037.9706726
ACCGTAC1700.037.9706727
GTACTAG1800.037.81738730
TAGACCT451.1006274E-437.7205544
ATGGCAA2250.037.5484370
GTATCAA16050.037.4447171
TACTAGA1750.036.97442231
TCTGGAG3250.036.82634770
CTAGATC1400.036.55758733
AGACCGG500.00212890635.9199969
ATGCCGT1750.035.60365344
Produced by FastQC (version 0.11.5)