FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337039
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42560.31831532214093977No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGGGATCAGTA39890.29834582237316937No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37230.27845111473936063No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35840.26805500811868616No Hit
CCCCTGTGTTGACTCAGCCACCCTCGGTGTCGGTGGCCCCAGGACAGACG34150.255415137479161No Hit
GTGTTGACTCAGCCACCCTCGGTGTCGGTGGCCCCAGGACAGACGGCCAA33420.24995531169995788No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32870.2458417443320651No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC31970.23911045227551328No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGGGATCAGTATCACTTTCCCACAG30950.2314816546114212No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29520.22078637945489996No Hit
CCCCAGGACAGACGGCCAAGATGACCTGTGGGGGAAATAACATTGCGACC28570.2136811267285397No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27940.20896922228895343No Hit
GTATAGTACTGGGGCCACCAACTACAACTCCTCCCTCAAGAGTCGAGTCA26980.20178917742863148No Hit
CCTATGATGTTAGCGGCCGGCCCCCAGGGATCCCTGAACGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC26890.20111604822297627No Hit
CTATTACTGTCAGCTGTGGGAAAGTGATACTGATCCCGTGGTTTTCGGCG26170.19573101457773484No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC26070.19498309323811797No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25160.18817700904760445No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25040.18727950344006422No Hit
CATCATAGGCGACCAACACAGGGGCCTGGCCTGACCTCTGCTGGTACCAG24410.18256759900047792No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24400.18249280686651623No Hit
GGCCAGGCCCCTGTGTTGGTCGCCTATGATGTTAGCGGCCGGCCCCCAGG24160.18069779565143576No Hit
ACACAGGGGCCTGGCCTGACCTCTGCTGGTACCAGTGCACACTTTTGGTC24090.18017425071370394No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23900.17875320016843188No Hit
CCCCTGTGTTGGTCGCCTATGATGTTAGCGGCCGGCCCCCAGGGATCCCT23630.17673381255146633No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23080.17262024518357355No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22930.17149836317414824No Hit
GTACTATACATGCGCCCAACCCACTCCAGGCCCTTCCCGGCGGGCTGCCGGATCCAGGTCCAGTAGTAAGTACCA22160.16573936885909835No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21830.16327122843836267No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21630.16177538575912895No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21540.16110225655347377No Hit
GGTCAGGCCAGGCCCCTGTGTTGGTCGCCTATGATGTTAGCGGCCGGCCC20760.15526847010446218No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20360.1522767847459947No Hit
CGGCCACCCTGACCATCAGCGGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGCTGTGGGAAAGTGATA20130.1505565656648759No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19930.14906072298564216No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCCGCTGATGGTCAGGGT19930.14906072298564216No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19100.14285297586682213No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCCCTGTGTTGACTCAGC18700.13986129050835466No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18180.13597209954234693No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18160.13582251527442357No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17790.13305520631784112No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCCCTGTGTTGACT17460.13058706589710548No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA16740.125202032251864No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGTTCAGGGATCCCTGGGGGCCGGCCGCTAACATCATAGG16500.12340702103678353No Hit
GGCCTGGCCTGACCTCTGCTGGTACCAGTGCACACTTTTGGTCGCAATGTTATTTCCCCCACAGGTCATCTTGGC16410.12273389183112833No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGC16020.11981699860662254No Hit
GCACAGGCTCTGTGACCTCCCCTGTGTTGACTCAGCCACCCTCGGTGTCG15870.11869511659719724No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15800.11817157165946543No Hit
GGCTCTGTGACCTCCCCTGTGTTGACTCAGCCACCCTCGGTGTCGGTGGCCCCAGGACAGACGGCCAAGATGAC15700.11742365031984857No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15630.11690010538211676No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT15610.11675052111419337No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15410.11525467843495962No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15270.11420758855949602No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15130.11316049868403241No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCCCTGTGTTGACTCAGCCAC14650.10957047625387144No Hit
GTGATACTGATCCCGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTA14430.10792504930671433No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT14170.10598045382371045No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14070.10523253248409359No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG14040.10500815608220852No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13990.10463419541240009No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13850.10358710553693648No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13830.10343752126901311No Hit
GTCCTGGGGCCACCGACACCGAGGGTGGCTGAGTCAACACAGGGGAGGTC13820.10336272913505141No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13770.10298876846524298No Hit
GGCCTGACCTCTGCTGGTACCAGTGCACACTTTTGGTCGCAATGTTATTTCCCCCACAGGTCATCTTGGCCGT13670.1022408471256261No Hit
CTGCTGGTACCAGTGCACACTTTTGGTCGCAATGTTATTTCCCCCACAGGTCATCTTGGCCGTCTGTCCTGGGGC13650.10209126285770273No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13440.1005206280445073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)