FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337039
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39160.29288599659396625No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38960.2913901539147325No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGGGATCAGTA37210.27830153047143724No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35570.2660356205017206No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC34000.25429325546973575No Hit
GTGTTGACTCAGCCACCCTCGGTGTCGGTGGCCCCAGGACAGACGGCCAA32960.2465148735377203No Hit
CCCCTGTGTTGACTCAGCCACCCTCGGTGTCGGTGGCCCCAGGACAGACG32690.24449548592075476No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31740.23739023319439448No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGGGATCAGTATCACTTTCCCACAG31000.23185561528122964No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC29200.218393031168126No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26970.20171438529466978No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26590.19887228420412567No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26550.19857311566827893No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26030.19468392470227122No Hit
CCTATGATGTTAGCGGCCGGCCCCCAGGGATCCCTGAACGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC25860.19341245842492252No Hit
CTATTACTGTCAGCTGTGGGAAAGTGATACTGATCCCGTGGTTTTCGGCG25510.1907947337362635No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25340.18952326745891482No Hit
GTATAGTACTGGGGCCACCAACTACAACTCCTCCCTCAAGAGTCGAGTCA25230.18870055398533625No Hit
CCCCAGGACAGACGGCCAAGATGACCTGTGGGGGAAATAACATTGCGACC24410.18256759900047792No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23580.1763598518816579No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23240.17381691932696056No Hit
CCCCTGTGTTGGTCGCCTATGATGTTAGCGGCCGGCCCCCAGGGATCCCT23130.172994205853382No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23000.17202190811188006No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22670.16955376769114439No Hit
GGCCAGGCCCCTGTGTTGGTCGCCTATGATGTTAGCGGCCGGCCCCCAGG22580.1688806384854892No Hit
ACACAGGGGCCTGGCCTGACCTCTGCTGGTACCAGTGCACACTTTTGGTC22380.16738479580625545No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21830.16327122843836267No Hit
GTACTATACATGCGCCCAACCCACTCCAGGCCCTTCCCGGCGGGCTGCCGGATCCAGGTCCAGTAGTAAGTACCA21360.1597559981421634No Hit
CATCATAGGCGACCAACACAGGGGCCTGGCCTGACCTCTGCTGGTACCAG20840.15586680717615567No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGTTCAGGGATCCCTGGGGGCCGGCCGCTAACATCATAGG20660.1545205487648453No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCCCTGTGTTGACTCAGC20460.15302470608561158No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20290.15175323980826289No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20030.14980864432525903No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19760.1477892567082935No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19710.14741529603848505No Hit
GGTCAGGCCAGGCCCCTGTGTTGGTCGCCTATGATGTTAGCGGCCGGCCC19440.14539590842151948No Hit
CGGCCACCCTGACCATCAGCGGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGCTGTGGGAAAGTGATA19000.14210505452720526No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18890.1412823410536267No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCCCTGTGTTGACT18540.13866461636496766No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18230.13634606021215537No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17980.1344762568631132No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17770.13290562204991777No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA17290.12931559961975678No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCCGCTGATGGTCAGGGT17210.12871726254806332No Hit
GGCCTGGCCTGACCTCTGCTGGTACCAGTGCACACTTTTGGTCGCAATGTTATTTCCCCCACAGGTCATCTTGGC16350.12228513902735821No Hit
GGCTCTGTGACCTCCCCTGTGTTGACTCAGCCACCCTCGGTGTCGGTGGCCCCAGGACAGACGGCCAAGATGAC15970.11944303793681411No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT15680.1172740660519252No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC15630.11690010538211676No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15520.11607739190853819No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15330.11465634136326615No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15210.11375883575572589No Hit
GGCCTGACCTCTGCTGGTACCAGTGCACACTTTTGGTCGCAATGTTATTTCCCCCACAGGTCATCTTGGCCGT14980.1120386166746071No Hit
GTGATACTGATCCCGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTA14960.11188903240668371No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC14590.10912172345010131No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGC14540.10874776278029287No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14520.1085981785123695No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14230.10642920662748058No Hit
GCACAGGCTCTGTGACCTCCCCTGTGTTGACTCAGCCACCCTCGGTGTCG14230.10642920662748058No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13950.10433502687655334No Hit
CTGCTGGTACCAGTGCACACTTTTGGTCGCAATGTTATTTCCCCCACAGGTCATCTTGGCCGTCTGTCCTGGGGC13790.10313835273316635No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT13740.10276439206335791No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13640.10201647072374104No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13640.10201647072374104No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG13590.1016425100539326No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC13400.10022145950866056No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)