FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794044
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39290.4948088519024135No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33070.41647566129836633No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA32080.4040078383565646No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26250.33058621436595453No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24290.3059024436932966No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23020.28990836779825807No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20890.26308365783256343No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20450.2575424031917627No Hit
GTAATAAACCCCAACATCTTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG20070.2527567741837984No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC18680.2352514470230869No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18670.23512550941761412No Hit
GTGCTGACTCAGTCTCCACTCTCCCTGTCCGTCACCCTTGGACAGCCGGC18430.23210300688626828No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA17810.22429487534695808No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17680.2226576864758124No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17310.21799799507332085No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17220.21686455662406617No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17190.21648674380764793No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17160.2161089309912297No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17010.21421986690913852No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16960.2135901788817748No Hit
GAACACAGTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA16930.21321236606535657No Hit
GTATCAACAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAATCGGGACTCTGGGGTCCCAGA16290.20515235931510092No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15840.1994851670688274No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGCCA15590.19633672693200882No Hit
CAAGGAACACAGTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT15280.19243266116235372No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15240.19192891074046275No Hit
GCTAATGCTCTGGGTCCCAGGATGCAGTGGCGATGCTGTGCTGACTCAGT14600.18386890399020708No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14310.1802167134314975No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13970.1759348348454242No Hit
GTCTCCACTCTCCCTGTCCGTCACCCTTGGACAGCCGGCCTCCATCTCCT13840.17429764597427852No Hit
GGTCTAGTCAAAGCCTCGTATTTGGCGATGGAAGCATCCACTTGAGTTGGTATCAACAGAGGCCAGGCCAATCTC13840.17429764597427852No Hit
GTTTATTACTGCATGCAAGGAACACAGTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA13720.17278639470860557No Hit
CTGTTGATACCAACTCAAGTGGATGCTTCCATCGCCAAATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGC13620.17152701865387812No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGCGATGCTGTGCTGACTCAGTCTCCACTCTCC13610.17140108104840537No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGCGATGCTGTGCTGA13220.16648951443496834No Hit
GTCTGGGACCCCAGAGTCCCGATTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGTTGATA13210.1663635768294956No Hit
GTGTTCCTTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGC12940.1629632614817315No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12740.1604445093722766No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12690.15981482134491287No Hit
GTCCAAGGGTGACGGACAGGGAGAGTGGAGACTGAGTCAGCACAGCATCG12680.15968888373944012No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12640.15918513331754916No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12240.15414762909863938No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12170.15326606586033015No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAATCGGGAC11750.14797668643047487No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11630.14646543516480195No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGCGATGCTGTGCTGACTCAGTCTCCACT11520.14508012150460176No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11510.14495418389912904No Hit
TTATTACTGCATGCAAGGAACACAGTGGCCGTGGACGTTCGGCCAAGGGA11370.1431910574225106No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11090.13966480446927373No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10920.13752386517623708No Hit
CTCCCTGTCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA10850.13664230193792787No Hit
CAGTAATAAACCCCAACATCTTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG10490.13210854814090905No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10440.13147886011354534No Hit
GGTATCAACAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTT10380.13072323448070888No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10350.13034542166429064No Hit
GTTGATACCAACTCAAGTGGATGCTTCCATCGCCAAATACGAGGCTTTGA10340.1302194840588179No Hit
CCACTCTCCCTGTCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAG10330.13009354645334514No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10300.12971573363692693No Hit
AGTCAAAGCCTCGTATTTGGCGATGGAAGCATCCACTTGAGTTGGTATCAACAGAGGCCAGGCCAATCTCCAAGG10250.1290860456095632No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10220.12870823279314497No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10170.12807854476578126No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10150.12782666955483576No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10000.12593760547274457No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10000.12593760547274457No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA9970.12555979265632633No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9930.12505604223443537No Hit
ACAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAATCGGGACTCTGGGGTCCCAGACAGATT9790.12329291575781695No Hit
ATGCTGTGCTGACTCAGTCTCCACTCTCCCTGTCCGTCACCCTTGGACAG9790.12329291575781695No Hit
GCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCT9610.12102603885930753No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGCGATG9460.11913697477721637No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9390.11825541153890717No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9360.11787759872248893No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9320.11737384830059795No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9290.11699603548417972No Hit
ACTGTGTTCCTTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACC9230.11624040985134326No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9210.11598853464039775No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9160.11535884661303404No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGCGATGCTGTGCTGACTCAGTCTC9020.11359572013641561No Hit
CCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGTTGATACCAA9010.11346978253094286No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8930.11246228168716092No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8840.1113288432379062No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8800.11082509281601523No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8790.11069915521054248No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8690.10943977915581504No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC8660.10906196633939681No Hit
GGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAATCGGGACTCTGG8540.10755071507372388No Hit
AATCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCAC8450.10641727662446918No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8450.10641727662446918No Hit
GATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAAGATGTTGGGGTTTATTACTGCATGCAAGGAACACAGTGG8370.1054097757806872No Hit
GTCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATTTGGCGATGGAAGCAT8310.10465415014785073No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8130.10238727324934135No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8040.10125383480008665No Hit
ATGTTGGGGTTTATTACTGCATGCAAGGAACACAGTGGCCGTGGACGTTC8010.1008760219836684No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7990.10062414677272292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTTA353.5178346E-758.56196615
CCGTTAA408.840543E-751.24494616
GTCGACG200.007651011551.2417214
ACCGTAC1300.047.36268227
GACCGTA1300.047.3537226
CGTATGC1400.044.37415741
CGTACTA1350.043.09372329
TCGTATG1450.042.78684640
TACCGCG405.3005002E-542.7014358
GTGGTAT1700.042.2229921
GTACTAG1400.041.57826630
GCCGTCT1350.041.15593746
CACGAGA1500.041.01920321
ACGAGAC1500.041.01920322
ATCTCGT1350.040.73869337
GATCTCG1400.039.26135336
TGCCGTC1600.039.00855645
CTAGATC1450.037.8308533
ACTAGAT1450.037.82367732
TACTAGA1450.037.79979331
Produced by FastQC (version 0.11.5)