FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794044
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39980.5034985466800328No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27460.34582466462815664No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA27430.3454468518117384No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25780.32466714690873555No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23040.2901602430092035No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21760.2740402295086922No Hit
GTAATAAACCCCAACATCTTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG21450.2701361637390371No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21390.26938053810620066No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC18380.23147331885890451No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17840.22467268816337635No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17720.2231614368977034No Hit
GTGCTGACTCAGTCTCCACTCTCCCTGTCCGTCACCCTTGGACAGCCGGC17190.21648674380764793No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17180.21636080620217518No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC16930.21321236606535657No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16860.21233080282704736No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16850.21220486522157464No Hit
GAACACAGTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA16580.20880454987381053No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16450.20716736100266486No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16280.2050264217096282No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA15520.19545516369369959No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGCCA15500.19520328848275412No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15480.19495141327180862No Hit
GTATCAACAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAATCGGGACTCTGGGGTCCCAGA15060.18966203384195335No Hit
GTGTTCCTTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGC15050.1895360962364806No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14790.18626171849418924No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14690.1850023424394618No Hit
GTTTATTACTGCATGCAAGGAACACAGTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA14660.18462452962304357No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14620.18412077920115258No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG14290.179964838220552No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14140.17807577413846085No Hit
GTCTGGGACCCCAGAGTCCCGATTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGTTGATA14100.17757202371656988No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13540.17051951781009617No Hit
CTGTTGATACCAACTCAAGTGGATGCTTCCATCGCCAAATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGC13200.16623763922402285No Hit
GCTAATGCTCTGGGTCCCAGGATGCAGTGGCGATGCTGTGCTGACTCAGT13020.16397076232551347No Hit
CAAGGAACACAGTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT12640.15918513331754916No Hit
GGTCTAGTCAAAGCCTCGTATTTGGCGATGGAAGCATCCACTTGAGTTGGTATCAACAGAGGCCAGGCCAATCTC12630.1590591957120764No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12580.15842950768471267No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGCGATGCTGTGCTGACTCAGTCTCCACTCTCC12550.15805169486829446No Hit
GTCTCCACTCTCCCTGTCCGTCACCCTTGGACAGCCGGCCTCCATCTCCT12330.15528106754789409No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12190.15351794107127564No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12020.15137700177823898No Hit
GTCCAAGGGTGACGGACAGGGAGAGTGGAGACTGAGTCAGCACAGCATCG11850.14923606248520233No Hit
TTATTACTGCATGCAAGGAACACAGTGGCCGTGGACGTTCGGCCAAGGGA11810.14873231206331136No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG11550.14545793432102No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGCGATGCTGTGCTGA11480.14457637108271076No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11330.1426873070006196No Hit
CTCCCTGTCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA11270.14193168136778314No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11130.14016855489116473No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11030.13890917883643727No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10690.13462730025036396No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10630.1338716746175275No Hit
CAGTAATAAACCCCAACATCTTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG10520.1324863609573273No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGCGATGCTGTGCTGACTCAGTCTCCACT10440.13147886011354534No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10350.13034542166429064No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10310.12984167124239968No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10220.12870823279314497No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10080.12694510631652653No Hit
GTTGATACCAACTCAAGTGGATGCTTCCATCGCCAAATACGAGGCTTTGA9970.12555979265632633No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9940.12518197983990811No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAATCGGGAC9900.12467822941801714No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA9880.12442635420707164No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9730.12253729012498048No Hit
ACAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAATCGGGACTCTGGGGTCCCAGACAGATT9610.12102603885930753No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9560.12039635083194382No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9480.11938884998816186No Hit
ACTGTGTTCCTTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACC9470.11926291238268912No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9330.1174997859060707No Hit
CCACTCTCCCTGTCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAG9170.11548478421850679No Hit
GGTATCAACAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTT9160.11535884661303404No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9040.1138475953473611No Hit
AGTCAAAGCCTCGTATTTGGCGATGGAAGCATCCACTTGAGTTGGTATCAACAGAGGCCAGGCCAATCTCCAAGG8790.11069915521054248No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8740.11006946718317877No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8720.10981759197223329No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8660.10906196633939681No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8640.10881009112845132No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG8620.10855821591750583No Hit
GGGTGGAGGCTGAAGATGTTGGGGTTTATTACTGCATGCAAGGAACACAGTGGCCGTGGACGTTCGGCCAAGGGA8600.10830634070656034No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8560.10780259028466938No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8560.10780259028466938No Hit
CCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGTTGATACCAA8440.10629133901899643No Hit
GCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCT8400.10578758859710545No Hit
GATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAAGATGTTGGGGTTTATTACTGCATGCAAGGAACACAGTGG8190.10314289888217781No Hit
GGGTCCCAGGATGCAGTGGCGATGCTGTGCTGACTCAGTCTCCACTCTCCCTGTCCGTCACCCTTGGACAGCCGG8190.10314289888217781No Hit
ATGCTGTGCTGACTCAGTCTCCACTCTCCCTGTCCGTCACCCTTGGACAG8180.10301696127670507No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8170.10289102367123232No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGCGATG8090.10188352282745038No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8070.10163164761650488No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7970.10037227156177743No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7960.10024633395630468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)