FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364444
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50100.3671825300268827No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44970.3295847979103576No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37900.2777688201201368No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31830.2332818349452231No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31460.23057010767755948No Hit
GTCTCAGTCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGG30830.22595284232991608No Hit
GATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCGGTGGATCTGGGACAGATTTCA30310.2221417661699564No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29340.21503264333310856No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27090.19854240994866776No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25820.18923458932722778No Hit
GTAGGAGACAGAGTCATCATCACTTGCCGGGCAAGTCAGACCATTACCCATTATTTAAATTGGTATCAGCAGAGA25450.1865228620595642No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25220.18483719375804358No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24950.18285836575191067No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23880.17501634365353214No Hit
CAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT23470.17201145668125625No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23310.17083881786280714No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22820.16724761148130668No Hit
ACTTACTACTGTCAACAGAGTTTCAGTCTTCCTCAAACCTTCGGCCAAGGGACACGACTGGACATTGAACGAA22740.1666612920720821No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC22720.16651471221977596No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22710.1664414222936229No Hit
GATTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTCTT22320.16358311517365315No Hit
GGACACGACTGGACATTGAACGAACTGTGGCTGCACCATCTGTCTTCATC22180.16255705620751018No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21980.16109125768444874No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAATT21940.1607980979798365No Hit
CGACTGGACATTGAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT21650.15867269012139745No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21600.15830624049063208No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCATCATCACTT20930.15339581543837635No Hit
GGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19960.14628669260152855No Hit
GTCGTGTCCCTTGGCCGAAGGTTTGAGGAAGACTGAAACTCTGTTGACAG19560.1433550955554057No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19440.14247561644156886No Hit
GCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18780.13763848131546624No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAG18500.13558636338318025No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18500.13558636338318025No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18340.13441372456473114No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18070.13243489655859822No Hit
CACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTCTTCCTCAAACCTT18040.13221502678013902No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17940.13148212751860833No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACCGCCA17800.13045606855246533No Hit
GTGCAGCCACAGTTCGTTCAATGTCCAGTCGTGTCCCTTGGCCGAAGGTT17030.1248127442386789No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16790.12305378601100521No Hit
GAGTGAAATCTGTCCCAGATCCACCGCCACTGAACCTTGATGGGACCCCA16380.12004889903872933No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16320.1196091594818109No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAATTTAAAT16240.11902284007258634No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16110.11807007103259642No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT16090.11792349118029029No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16070.11777691132798414No Hit
AGTCAGACCATTACCCATTATTTAAATTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATG15750.11543163369108589No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAGGTT15200.11140068775266701No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG14980.10978830937729948No Hit
GTTCAGTGGCGGTGGATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTA14880.10905541011576876No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14320.10495117425119684No Hit
GTCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCC14200.10407169513735998No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14180.10392511528505384No Hit
GTTTCAGTCTTCCTCAAACCTTCGGCCAAGGGACACGACTGGACATTGAA14150.10370524550659461No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT14050.10297234624506392No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATT14040.10289905631891084No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13870.10165312757430865No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13780.10099351823893102No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13730.10062706860816566No Hit
CTGCAAACTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTC13660.10011403912509417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT1803.6379788E-1241.5075570
CGTATGC1450.040.4415341
TCGTATG1400.039.3408740
GTATCAA40800.036.6944771
ACGTAGA3300.035.84692427
GACGTAG3300.035.82586726
AGACGTA3350.035.26784525
CGAGACG3550.034.2142323
ATGTCGT400.002710853734.1980537
CTAACGC701.1653046E-633.7167783
CGTAGAG3550.033.3433228
GAGACGT3550.033.26627724
ACGAGAC3850.032.41501622
TATCAAC46750.031.9498022
ATCAACG47000.031.6363833
CCCACGA3750.031.4747619
TCAACGC47700.031.2428024
CAACGCA48150.031.1608855
AACGCAG48300.031.064116
ATCTCCG3950.030.72696510
Produced by FastQC (version 0.11.5)