FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364444
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53310.3907085963220184No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38340.28099357687087195No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35180.2578339602065017No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34670.2540961739726951No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33800.24771995039737796No Hit
GTCTCAGTCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGG31400.23013036812064108No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30180.2211889971299665No Hit
GATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCGGTGGATCTGGGACAGATTTCA29600.2169381814130884No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28870.21158801680391426No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27470.20132742714248442No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26070.19106683748105455No Hit
GTAGGAGACAGAGTCATCATCACTTGCCGGGCAAGTCAGACCATTACCCATTATTTAAATTGGTATCAGCAGAGA25380.1860098325764927No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24690.18095282767193083No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24150.17699517165966502No Hit
CAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT22410.16424272450903077No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22090.16189744687213253No Hit
GATTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTCTT21940.1607980979798365No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21940.1607980979798365No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21850.16013848864445884No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21400.1568404419675707No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21370.15662057218911146No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCATCATCACTT21310.15618083263219304No Hit
GGACACGACTGGACATTGAACGAACTGTGGCTGCACCATCTGTCTTCATC21200.15537464344450927No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20780.1522964665460803No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20690.1516368572107027No Hit
CGACTGGACATTGAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT20610.1510505378014781No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20590.15090395794917197No Hit
ACTTACTACTGTCAACAGAGTTTCAGTCTTCCTCAAACCTTCGGCCAAGGGACACGACTGGACATTGAACGAA20570.15075737809686585No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAATT20070.1470928817892123No Hit
GGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20020.14672643215844697No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19760.14482089407846713No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19130.14020362873082368No Hit
GTCGTGTCCCTTGGCCGAAGGTTTGAGGAAGACTGAAACTCTGTTGACAG19090.1399104690262114No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19070.13976388917390528No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18670.13683229212778245No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACCGCCA18440.13514662382626183No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAG18170.13316779582012891No Hit
CACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTCTTCCTCAAACCTT17640.1292834297340162No Hit
GTGCAGCCACAGTTCGTTCAATGTCCAGTCGTGTCCCTTGGCCGAAGGTT17560.12869711032479164No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16740.12268733638023986No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16140.11828994081105565No Hit
GAGTGAAATCTGTCCCAGATCCACCGCCACTGAACCTTGATGGGACCCCA16000.11726388184491265No Hit
AGTCAGACCATTACCCATTATTTAAATTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATG15930.11675085236184117No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAATTTAAAT15830.11601795310031046No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAGGTT15560.11403912509417756No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCAATGTCCAGTCGTGTCCCTTGGCCGAAGGTTTGAGGAAGA15230.11162055753112622No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15060.11037462878652404No Hit
GCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15050.11030133886037097No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14990.10986159930345253No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14830.10868896048500341No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14760.10817593100193193No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG14550.10663684255271745No Hit
GTTCAGTGGCGGTGGATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTA14460.1059772332173398No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT14290.10473130447273761No Hit
GTCCAGTCGTGTCCCTTGGCCGAAGGTTTGAGGAAGACTGAAACTCTGTT14150.10370524550659461No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCGGTGGATCTGGGACAGATTTCACT14130.10355866565428849No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14030.1028257663927578No Hit
GTAATATATGGCCGTGTCCGCAGGGGTCACTGACGTCACCTTCAGGGAGA13980.10245931676199244No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13950.10223944698353321No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13920.10201957720507401No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13800.10114009809123717No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13770.10092022831277796No Hit
GTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGCCGGGCAAGTCAGA13730.10062706860816566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC551.7814455E-742.8483853
CCTAACG504.539743E-640.408792
AATGGTA10350.036.6412370
GTATCAA42450.036.5047951
GTGGTAT4750.033.3329731
CGACCGG2150.032.88123311
TATCAAC47450.032.36112
ATCAACG48250.031.6779923
TCAACGC48750.031.4900554
CAACGCA48900.031.3934565
AACGCAG49400.031.2120086
TGTTAGA17350.030.3583770
CGACGAA550.005678165729.40351968
ACGCAGA52250.029.3162427
CGCAGAG52500.029.1766388
AGAGTAC53250.028.57498711
GTGCTAG4450.027.2528721
CGCCTAG500.00867968326.95599219
ATAAGAC500.00871545826.9332683
CCGACCG2500.026.93228110
Produced by FastQC (version 0.11.5)