FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793347
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17720.22335749678261846No Hit
CCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA16710.21062662365900423No Hit
GTAATAAACGGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGAGA16280.20520654896281199No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCGC15520.19562688205791412No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14490.18264391243680256No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13060.16461901286574473No Hit
AAGTAGTACTCTACGTAGCAATTATAACCACAAGATTCCGGTTTTTGCATCTCCGGATAGCAGCTGGGACTACTA11830.14911507826966006No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11820.1489890300209114No Hit
GTTTATTACTGTGCGAGACAGGGCCGATATTGTAGTAGTCCCAGCTGCTA11790.14861088527466543No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11690.14735040278717887No Hit
ATATTGTAGTAGTCCCAGCTGCTATCCGGAGATGCAAAAACCGGAATCTTGTGGTTATAATTGCTACGTAGAGTA11680.14722435453843022No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCGCAGTGTTGTCGTCCCACGCCTGACAGTAATAGTCAG11140.1404177491060028No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11130.14029170085725415No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10640.13411533666856998No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC10540.13285485418108345No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10370.13071203395235628No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10170.12819106897738317No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10160.12806502072863452No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC10040.12655244174365066No Hit
CTATTACTGTCAGGCGTGGGACGACAACACTGCGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC9610.12113236704745843No Hit
GACTATTACTGTCAGGCGTGGGACGACAACACTGCGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG9390.11835930557498799No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACGACAACACTGCGATATTCGGCGGAGGGACCAAG9310.11735091958499874No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9300.11722487133625009No Hit
GAACAGGGGGCTGGCCTGACTTTTGTTGATACCAGCAAGCATATTTATCC9250.1165946300925068No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9070.114325761615031No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9000.11344342387379042No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8830.11130060364506326No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8820.11117455539631461No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCACCATCACCTGCTCAGC8790.11079641065006865No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8760.11041826590382266No Hit
CCCCAGGACAGACAGCCACCATCACCTGCTCAGCAGATAAATTGGGGGAT8620.10865359042134148No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8480.1068889149388603No Hit
CTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCA8260.10411585346638985No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8160.10285537097890331No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG8140.10260327448140599No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8050.10146884024266808No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG8020.10109069549642212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)