FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793347
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30060.37890103573845996No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16820.21201315439523938No Hit
GTAATAAACGGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGAGA16220.20445025947032006No Hit
CCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA14590.18390439492428912No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14260.17974480271558346No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCGC13540.17066932880568023No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13050.16449296461699608No Hit
GTTTATTACTGTGCGAGACAGGGCCGATATTGTAGTAGTCCCAGCTGCTA11550.1455857273046977No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11540.14545967905594903No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10910.1375186393847837No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10410.1312162269473509No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCGCAGTGTTGTCGTCCCACGCCTGACAGTAATAGTCAG10230.1289473584698751No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10230.1289473584698751No Hit
AAGTAGTACTCTACGTAGCAATTATAACCACAAGATTCCGGTTTTTGCATCTCCGGATAGCAGCTGGGACTACTA10170.12819106897738317No Hit
ATATTGTAGTAGTCCCAGCTGCTATCCGGAGATGCAAAAACCGGAATCTTGTGGTTATAATTGCTACGTAGAGTA10090.1271826829873939No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10040.12655244174365066No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9960.1255440557536614No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9730.1226449460324423No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC9590.12088027054996112No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC9530.12012398105746917No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9440.11898954681873128No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG9370.11810720907749067No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9320.1174769678337474No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9130.11508205110752293No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9020.11369552037128772No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8970.11306527912754445No Hit
CTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCA8960.11293923087879577No Hit
GACTATTACTGTCAGGCGTGGGACGACAACACTGCGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG8780.11067036240131997No Hit
GAACAGGGGGCTGGCCTGACTTTTGTTGATACCAGCAAGCATATTTATCC8690.10953592816258208No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8470.10676286669011165No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACGACAACACTGCGATATTCGGCGGAGGGACCAAG8380.10562843245137372No Hit
CTATTACTGTCAGGCGTGGGACGACAACACTGCGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC8370.10550238420262507No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8350.10525028770512776No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8110.10222512973516003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA200.00806520350.5628369
TACTGAT6800.036.81968770
CGATATC400.00244167534.9332846
TTTCGTG450.00325893232.9349766
ATCAACG23550.032.626813
GTGGTAT2900.032.5605661
TCAACGC23750.032.3581734
AACGCAG24400.031.7744586
CAACGCA24400.031.498165
ATAGAAC553.7361804E-430.6364153
ACCGACT450.005143878530.00289226
TTTCACG450.005143878530.00289226
GTATACT450.005178911629.9612734
ACGCAGA26450.029.311797
CGCAGAG26500.029.129288
GTATCAA27050.028.4249341
CAGAGTA27250.028.08015310
GCAGAGT27800.027.7671229
AGTTTAT3050.027.626482
GTGGTGA4950.027.45802970
Produced by FastQC (version 0.11.5)