FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004692275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647717
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43660.2649726864504038No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42320.2568402219555907No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAACTTCAGGGAGA40460.2455518757165217No Hit
ATATTACTGTCAGCAGTATGGGAGCTCAGTGTGGACGTTCGGCCAAGGGA34280.2080454349867119No Hit
GTATATTACTGTCAGCAGTATGGGAGCTCAGTGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA33700.20452541304119579No Hit
GTCTCTGGTGGTTCCGTCAGCAGTGGCAGTTACTACTGGAGCTGGGTCCG30790.18686461328007176No Hit
GTGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG29800.180856299959277No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA27910.16938588361957788No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27780.1685969131835139No Hit
GTGTATTACTGTGCGAGGAGCCGTTACTATGATAGTGGTGGTTACCCACC27400.16629069190886542No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25220.15306026459640823No Hit
GTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGGGTGAAGTCAGTCCCTGACCCACTGCCA24760.15026852305341268No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24200.1468698811749833No Hit
CTCCTGCAGGGCCAGTCAGAATGTTGGCAGCAGCTACTTAGCCTGGTACC24180.14674850110789656No Hit
CACCTGCACTGTCTCTGGTGGTTCCGTCAGCAGTGGCAGTTACTACTGGA23800.1444422798332481No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23770.1442602097326179No Hit
CAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGGGTGAAGTCAGTCCCTGACCCACTGCCA23340.1416505382902525No Hit
GTATGGGAGCTCAGTGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT23160.14055811768647164No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23110.14025466751875473No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22460.13630981533843495No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22250.13503532463402393No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22200.13473187446630702No Hit
CGTCCACACTGAGCTCCCATACTGCTGACAGTAATATACTGCAAAATCTT22000.1335180737954394No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21460.1302408119840968No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21340.12951253158157622No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC21040.12769183057527475No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20920.12696355017275418No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20490.12435387873038876No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTGGCAG20200.12259386775763072No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20020.12150144715384985No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18990.11525037369888155No Hit
GTATTACTGTGCGAGGAGCCGTTACTATGATAGTGGTGGTTACCCACCCAGTTTTGACTTCTGGGGCCAGGGAAC18570.11270139229005952No Hit
CAGTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTGCTGGGCGTGCCTA18500.11227656205525585No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18480.1121551819881691No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17860.10839239990847943No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17620.10693583910343826No Hit
ATGCTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCAGGGACTGACTTCACCCTCA17420.10572203843257065No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17170.10420478759398609No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16950.1028696068560317No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16870.10238408658768466No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16790.10189856631933761No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16780.10183787628579423No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16630.1009275257826435No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16520.1002599354136663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)