FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647717
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48200.292525961679099No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41220.2501643182658187No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAACTTCAGGGAGA40560.24615877605195552No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35910.2179379104542831No Hit
GTGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG33420.20282609210198113No Hit
GTATATTACTGTCAGCAGTATGGGAGCTCAGTGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA32050.19451155750653784No Hit
ATATTACTGTCAGCAGTATGGGAGCTCAGTGTGGACGTTCGGCCAAGGGA30600.18571150264274752No Hit
GTCTCTGGTGGTTCCGTCAGCAGTGGCAGTTACTACTGGAGCTGGGTCCG30160.18304114116683873No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29450.17873214878525864No Hit
GTGTATTACTGTGCGAGGAGCCGTTACTATGATAGTGGTGGTTACCCACC27510.1669582822778426No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA25920.15730856694444495No Hit
GTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGGGTGAAGTCAGTCCCTGACCCACTGCCA25300.15354578486475529No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24030.14583815060474584No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22810.1384339665124533No Hit
CAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGGGTGAAGTCAGTCCCTGACCCACTGCCA22540.13679533560678198No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22460.13630981533843495No Hit
CTCCTGCAGGGCCAGTCAGAATGTTGGCAGCAGCTACTTAGCCTGGTACC22080.13400359406378642No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21870.13272910335937543No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21810.13236496315811513No Hit
CACCTGCACTGTCTCTGGTGGTTCCGTCAGCAGTGGCAGTTACTACTGGA21670.1315153026885078No Hit
GTATGGGAGCTCAGTGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT20930.12702424020629755No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20920.12696355017275418No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20460.12417180862975861No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC20030.12156213718739323No Hit
CGTCCACACTGAGCTCCCATACTGCTGACAGTAATATACTGCAAAATCTT19630.11913453584565796No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19610.1190131557785712No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19540.11858832554376754No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18600.11288346239068967No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18520.11239794212234261No Hit
GTATTACTGTGCGAGGAGCCGTTACTATGATAGTGGTGGTTACCCACCCAGTTTTGACTTCTGGGGCCAGGGAAC18470.1120944919546257No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18340.11130552151856175No Hit
CAGTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTGCTGGGCGTGCCTA18300.11106276138438821No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTGGCAG18210.11051655108249778No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18160.11021310091478087No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18080.10972758064643383No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17990.1091813703445434No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17890.10857447000910958No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17380.10547927829839712No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16880.10244477662122804No Hit
ATGCTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCAGGGACTGACTTCACCCTCA16880.10244477662122804No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16830.10214132645351114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA350.0035100562.39220470
GTGGTAT5150.043.7991871
GGTATCA18650.042.782681
TACTGAT16500.041.46875870
TGGTATC5850.036.8127672
GTATCAA48500.036.5819931
AGAGTCG400.00293656433.64508412
TATCAAC54350.032.3799742
ATCAACG55300.031.6382883
TCAACGC55800.031.3566974
CTAACGC652.7559076E-531.0579433
CAACGCA56450.031.0571275
AACGCAG57200.030.7087386
CGCTTGC500.00480400630.42693967
CGCAGAG59200.029.4439188
ACGCAGA59850.029.2928127
CCTACTG13450.028.89464268
GCCTACT13050.028.85313267
CCTAACG704.5672816E-528.8421442
AGAGTAC60650.028.51632111
Produced by FastQC (version 0.11.5)