FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510366
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA68360.4526055274019674No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT65570.43413318361244896No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG57480.3805700075345976No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCACCTTATGATGTACACTGGTACCAGCACCTTCCAGGAACAGC53180.3521000869987804No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC44720.2960871735724983No Hit
CCTTATGATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAATCTCCTCATCTATGGTAACACGAATCGG44500.2946305729869449No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC44230.29284292681376567No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC43000.28469920535817145No Hit
ACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATTCATTCAGAC42870.2838384868303444No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC42660.2824480953623162No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC38690.25616307570482916No Hit
GCTCCAGACTGAGGATGAGGCTGATTATTATTGCCAGTCCTATGACAGCAGTCTGAATGAATATGTCTTCGGAA37630.2491449092471626No Hit
GTCATAGGACTGGCAATAATAATCAGCCTCATCCTCAGTCTGGAGCCCAG37090.24556961690080417No Hit
GTCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGCCAGGGACACCTGGG35550.23537341280193014No Hit
GTCCTATGACAGCAGTCTGAATGAATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTACGTCAGCCCAAGGC34220.22656760017108435No Hit
GACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATTCATTCAGA32450.2148485863691317No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCTTGGTC30410.20134192639399986No Hit
TCCAGGAACAGCCCCCAATCTCCTCATCTATGGTAACACGAATCGGCGCCCAGGTGTCCCTGGCCGATTCTCTGG30170.19975290757339612No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG29760.1970383337548647No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG29150.1929995775858302No Hit
GATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAATCTCCTCAT28670.1898215399446227No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT28650.18968912170957236No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGCC28550.18902703053432082No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28550.18902703053432082No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27320.1808833090787266No Hit
GTTCCTGGAAGGTGCTGGTACCAGTGTACATCATAAGGTGCCCCGATGTT27070.1792280811405977No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC26670.17657971643959144No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG26460.1751893249715632No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26260.17386514262106006No Hit
GCCTTGGGCTGACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATTCATTCAGACTGCTGTCATAGGA25800.17081952321490287No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT25770.17062089586232743No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG25530.16903187704172368No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG25280.16737664910359476No Hit
GGCCATCACTGGGCTCCAGACTGAGGATGAGGCTGATTATTATTGCCAGT25090.1661186758706168No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA21710.14373999414711403No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG21560.14274685738423668No Hit
GGATGAGGCTGATTATTATTGCCAGTCCTATGACAGCAGTCTGAATGAATATGTCTTCGGAACTGGGACCAAGGT21360.14142267503373354No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCACCTTATGATGTACACTGGTACCAG21230.14056195650590653No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA21010.13910535592035309No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC20460.1354638544564695No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC20410.1351328088688437No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC19530.12930640652662997No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT19090.1263932053555231No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG19010.12586353241532186No Hit
TCACTGGGCTCCAGACTGAGGATGAGGCTGATTATTATTGCCAGTCCTAT18840.1247379774173942No Hit
GTGCTGGTACCAGTGTACATCATAAGGTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTG18740.12407588624214264No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG18660.12354621330194138No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGACTGAGGATG18550.12281791300916467No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG18290.1210964759535106No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18150.12016954830815843No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT18000.11917641154528108No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17710.11725634713705155No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG17580.11639562860922452No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC17580.11639562860922452No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT17550.11619700125664904No Hit
GTGTACATCATAAGGTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGA17500.11586595566902327No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC17410.11527007361129687No Hit
TCATAAGGTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACA17370.11500523714119623No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17250.11421072773089437No Hit
GTACCAGCACCTTCCAGGAACAGCCCCCAATCTCCTCATCTATGGTAACACGAATCGGCGCCCAGGTGTCCCTGG16970.11235687244018999No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG16600.10990713509175921No Hit
GATTATTATTGCCAGTCCTATGACAGCAGTCTGAATGAATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTA16320.10805327980105484No Hit
CATCTATGGTAACACGAATCGGCGCCCAGGTGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC16250.10758981597837876No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG16210.10732497950827811No Hit
GCCTCATCCTCAGTCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC16150.10692772480312719No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15880.10514007862994798No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC15610.10335243245676874No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGCCAGGGACACCTGGGCGCCGATT15550.10295517775161783No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15480.10249171392894173No Hit
GCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAAT15290.10123374069596375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1500.043.5626440
TCTCGTA1350.043.1289338
CGTATGC1550.042.23861741
TTGCGAA350.00118527940.4733953
ACCGTAC1450.039.61944627
GTATCAA26700.039.2054941
GTGGTAT3300.038.883571
TACGTAT800.001824023737.071670
ATGCCGT1750.035.64715244
GACCGTA1650.034.8009426
TATCAAC30300.034.2085532
TAGATCT1700.034.04244234
ACTAGAT2000.033.9585332
ATCTCGT1750.033.21843737
ATCAACG31950.032.5475923
TCAACGC31900.032.4906124
CACGCGG450.003568914532.33176461
GTACTAG1800.031.998430
CAACGCA32550.031.84185
ATGGCAA4450.031.65664770
Produced by FastQC (version 0.11.5)