FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510366
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT59310.39268627604170114No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA59260.3923552304540754No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCACCTTATGATGTACACTGGTACCAGCACCTTCCAGGAACAGC51810.343029437897834No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC50490.33428983438451343No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG48580.32164389293720863No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG47670.3156188632424194No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC45820.30337017650026554No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC45310.2999935115064825No Hit
CCTTATGATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAATCTCCTCATCTATGGTAACACGAATCGG44060.291717371815838No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC40410.267551043919156No Hit
GCTCCAGACTGAGGATGAGGCTGATTATTATTGCCAGTCCTATGACAGCAGTCTGAATGAATATGTCTTCGGAA37430.24782072689665946No Hit
GTCCTATGACAGCAGTCTGAATGAATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTACGTCAGCCCAAGGC36110.2390811233833389No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC35990.238286613973037No Hit
ACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATTCATTCAGAC34220.22656760017108435No Hit
GTCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGCCAGGGACACCTGGG33970.22491237223295546No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG33030.21868871518559077No Hit
GTCATAGGACTGGCAATAATAATCAGCCTCATCCTCAGTCTGGAGCCCAG32430.2147161681340814No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28550.18902703053432082No Hit
TCCAGGAACAGCCCCCAATCTCCTCATCTATGGTAACACGAATCGGCGCCCAGGTGTCCCTGGCCGATTCTCTGG28410.18810010288896864No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG28360.18776905730134286No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCTTGGTC28060.18578278377558816No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT27910.1847896470127108No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG27870.1845248105426102No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC27630.18293579172200647No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT27230.1802874270210002No Hit
GGCCATCACTGGGCTCCAGACTGAGGATGAGGCTGATTATTATTGCCAGT27030.17896324467049707No Hit
GATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAATCTCCTCAT26990.17869840820039645No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC26640.176381089087016No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26200.1734678879159091No Hit
GACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATTCATTCAGA25670.16995880468707586No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG24940.16512553910773944No Hit
GCCTTGGGCTGACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATATTCATTCAGACTGCTGTCATAGGA24850.16452965705001305No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGCC24700.1635365202871357No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC24700.1635365202871357No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG24000.15890188206037478No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23820.15771011794492196No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT22270.14744770472852275No Hit
GTTCCTGGAAGGTGCTGGTACCAGTGTACATCATAAGGTGCCCCGATGTT22220.147116659140897No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCACCTTATGATGTACACTGGTACCAG22130.14652077708317057No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA21680.14354136679453855No Hit
GGATGAGGCTGATTATTATTGCCAGTCCTATGACAGCAGTCTGAATGAATATGTCTTCGGAACTGGGACCAAGGT21220.14049574738838136No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20520.13586110916162042No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT20510.13579490004409525No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA20510.13579490004409525No Hit
TCACTGGGCTCCAGACTGAGGATGAGGCTGATTATTATTGCCAGTCCTAT19990.13235202593278717No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC19910.1318223529925859No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT19740.13069679799465825No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC19680.1302995432895073No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC19550.12943882476168028No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG19200.12712150564829983No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18350.12149373065866154No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT17950.1188453659576553No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC17950.1188453659576553No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17930.11871294772260498No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC17600.11652804684427483No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGACTGAGGATG17390.11513765537624655No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17370.11500523714119623No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG17160.11361484567316796No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG16960.11229066332266484No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC16830.11142994479483781No Hit
GTGCTGGTACCAGTGTACATCATAAGGTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTG16570.10970850773918375No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC16520.10937746215155797No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC16460.10898020744640703No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG16290.10785465244847939No Hit
GTACCAGCACCTTCCAGGAACAGCCCCCAATCTCCTCATCTATGGTAACACGAATCGGCGCCCAGGTGTCCCTGG16120.10672909745055172No Hit
CATCTATGGTAACACGAATCGGCGCCCAGGTGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC15920.1054049151000486No Hit
ATGGTAACACGAATCGGCGCCCAGGTGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG15870.10507386951242283No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15760.10434556921964609No Hit
GATTATTATTGCCAGTCCTATGACAGCAGTCTGAATGAATATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTA15690.10388210539697001No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG15550.10295517775161783No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15490.10255792304646688No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG15210.1007040677557625No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15140.10024060393308643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT851.07320375E-1071.87244470
AACGTCG200.00802970350.6215226
AATGGTA6600.043.96742270
GTGGTAT3250.041.5273481
GTACGTA1009.385985E-1041.0919669
AGACTAA800.001577805438.1822470
GTATCAA31450.036.905861
TATCAAC34650.033.1856042
ATCAACG35850.032.2597053
TCAACGC36000.031.9390264
CAACGCA36350.031.9118965
AACGCAG36650.031.5597176
GGTATCA17800.031.2767431
GGTACGT1151.3511453E-831.27631868
TGGTATC4400.030.6553252
ACGCAGA38000.030.3497737
CGCAGAG38300.030.1120478
CCTAACG450.005169553729.9740942
CTTAGAC450.005172065529.9711153
TTGCCGA604.4127053E-429.78038456
Produced by FastQC (version 0.11.5)