FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191247
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45530.3822045302107791No Hit
GTAATATATGGCCGTATCCTCGGCTCTCAAACTGTCCATTTGCAGATACA41890.3516483147491662No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40610.34090327194947817No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG30610.2569576250769152No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG29590.24839516909591378No Hit
CTACAAACTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACG29530.24789149521467843No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29520.24780754956780582No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT29060.24394604981166795No Hit
CCCCTGGAGAGCCGGCCTCCGTCTCCTGCAGGTCTAGTCAGAGCCTCCTG25340.21271826917507453No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25150.21112330188449582No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24760.20784942165646586No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA23670.1986993461473565No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23340.19592913980056195No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23110.193998389922493No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22520.18904559675701177No Hit
GTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATGCACTGCCA22150.18593960782272695No Hit
GTTCAGTGGCAGTGCATCAGGCACAGATTTTACACTGAAAATCAGCAGAG21590.18123865159786343No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT21530.18073497771662803No Hit
GGTCTAGTCAGAGCCTCCTGCAGAGTAATGGAAACAACTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGGCTC21010.17636980407925476No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20840.1749427280824212No Hit
GCTATAGCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGG20440.17158490220751868No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20370.17099728267941072No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19020.15966462035161474No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT18710.1570623052985653No Hit
GTCCCTGACAGGTTCAGTGGCAGTGCATCAGGCACAGATTTTACACTGAA18580.15597101188922197No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18540.1556352293017317No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17780.14925536013941693No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17530.14715671896760285No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17360.1457296429707693No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17350.1456456973238967No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17250.14480624085517108No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17120.14371494744582777No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17010.14279154533022959No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16760.1406929041584155No Hit
CCCTAAGACTCTCCTGTGTAGCCTCTGGATTCACCTTGAGCAGCTATAGC16570.1390979368678368No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAAGCCAAATAGATCAGGAGCTGTGGAGCCTGCCCTGGCTTCTGCAGGTA16340.13716718698976788No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15410.1293602418306195No Hit
CAGTAATATATGGCCGTATCCTCGGCTCTCAAACTGTCCATTTGCAGATA15060.1264221441900798No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14830.12449139431201085No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG14690.12331615525579497No Hit
ATATATTACTGTGCGAAAGATCGAAGACCGGACGGACGCTGGAATTTTGA14680.12323220960892241No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14510.12180513361208885No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14490.12163724231834372No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14340.12037805761525527No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14310.12012622067463759No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCGTCTCCT14250.1196225467934022No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGA14140.11869914467780403No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAAGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT14120.1185312533840589No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG13870.11643261221224482No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13800.11584499268413688No Hit
GTGTAAAATCTGTGCCTGATGCACTGCCACTGAACCTGTCAGGGACCCCG13740.1153413188029015No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC13670.11475369927479355No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC13660.11466975362792099No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA13500.11332662327795998No Hit
AGCCAGGGCAGGCTCCACAGCTCCTGATCTATTTGGCTTCTAATCGGGCC13250.11122798210614591No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA13170.11055641693116541No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCAGCACAATATCC13080.10980090610931234No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCGTCTCCTGCAGGTCTA13030.10938117787494953No Hit
CAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATGCACTGCCA12950.10870961269996904No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACTCTCC12560.10543573247193906No Hit
GTATACATTACCACTACCGGTCAAACTTGAGACCCACTCCAGCCCCTTCC12550.1053517868250665No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12530.10518389553132138No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC12440.1044283847094683No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC12220.10258158047827193No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12160.10207790659703656No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT12080.10140634142205605No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11950.10031504801271272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAA200.00787679350.86768326
GTGGTAT1750.046.4490051
GGTATCA7200.046.0995641
TCGTATG1500.045.86755840
CGTATGC1600.045.2396341
TGGTATC1750.044.5061532
CTAGATC1400.043.83123433
CGTACTA1800.043.4065629
ACCGTAC1900.042.85035727
GACCGTA1950.041.73758726
GTATCAA20750.041.2957761
GCCGTCT1650.040.07956746
TGCCGTC1650.039.9898345
CGGCAAA700.001261013339.96273870
ATGCCGT1650.039.92450744
TAGATCT1550.039.61964434
ACTAGAT1750.038.93805332
TCTGGAG15050.038.5686970
GATCTCG1700.038.21634736
GTATGCC1900.038.168342
Produced by FastQC (version 0.11.5)