FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191247
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42430.35618137968028457No Hit
GTAATATATGGCCGTATCCTCGGCTCTCAAACTGTCCATTTGCAGATACA39120.3283953705654663No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36890.30967549131288474No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG32240.270640765517143No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31640.26560402670478916No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT28700.24092400652425566No Hit
CTACAAACTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACG26500.22245596421229183No Hit
CCCCTGGAGAGCCGGCCTCCGTCTCCTGCAGGTCTAGTCAGAGCCTCCTG25950.2178389536343009No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA25160.21120724753136838No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24940.209360443300172No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24590.20642234565963227No Hit
GTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATGCACTGCCA24170.20289662849098467No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22940.19257131392565943No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22370.18778641205392332No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22350.1876185207601782No Hit
GGTCTAGTCAGAGCCTCCTGCAGAGTAATGGAAACAACTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGGCTC21030.1765376953729999No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20600.17292803255747968No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20590.1728440869106071No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19110.1604201311734678No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT19080.16016829423285012No Hit
GTTCAGTGGCAGTGCATCAGGCACAGATTTTACACTGAAAATCAGCAGAG18980.1593288377641245No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18690.15689441400482015No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18410.15454393589238838No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17850.14984297966752486No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17580.14757644720196567No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17470.14665304508636748No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17340.14556175167702415No Hit
GTCCCTGACAGGTTCAGTGGCAGTGCATCAGGCACAGATTTTACACTGAA17000.14270759968335703No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16940.14220392580212166No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16920.1420360345083765No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16880.14170025192088626No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16660.13985344768968988No Hit
GCTATAGCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGG16340.13716718698976788No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15090.1266739811306975No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15070.12650608983695236No Hit
CCCTAAGACTCTCCTGTGTAGCCTCTGGATTCACCTTGAGCAGCTATAGC14990.12583452466197187No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14990.12583452466197187No Hit
CAGTAATATATGGCCGTATCCTCGGCTCTCAAACTGTCCATTTGCAGATA14860.12474323125262854No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14450.12130145973085348No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAAGCCAAATAGATCAGGAGCTGTGGAGCCTGCCCTGGCTTCTGCAGGTA14410.12096567714336322No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14280.1198743837340199No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAAGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT13900.11668444915286251No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13770.11559315574351918No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13690.11492159056853868No Hit
GTGTAAAATCTGTGCCTGATGCACTGCCACTGAACCTGTCAGGGACCCCG13600.1141660797466856No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA13530.11357846021857766No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG13430.11273900374985203No Hit
ATATATTACTGTGCGAAAGATCGAAGACCGGACGGACGCTGGAATTTTGA13280.1114798190467636No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13250.11122798210614591No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG13210.11089219951865566No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC13020.10929723222807695No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC12970.10887750399371414No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC12950.10870961269996904No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCGTCTCCTGCAGGTCTA12850.10787015623124338No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA12820.10761831929062571No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12770.10719859105626289No Hit
CAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATGCACTGCCA12640.10610729764691958No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCGTCTCCT12630.106023352000047No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGA12540.10526784117819395No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACTCTCC12470.104680221650086No Hit
GTATACATTACCACTACCGGTCAAACTTGAGACCCACTCCAGCCCCTTCC12350.10367287388761526No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12300.10325314565325244No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12250.10283341741888963No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12230.1026655261251445No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCAGCACAATATCC12100.10157423271580117No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCGATCACCTTCGGCCAAGGGA12030.10098661318769322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG14300.044.7485370
GGTATCA9350.043.352661
TCCGAGA6750.043.0911870
GTGGTAT2550.039.739941
GTATCAA22400.037.398121
CTACCGA400.00285718433.83294724
TGGTATC2850.033.1724052
AATGGTA3400.032.0807570
TATCAAC25950.032.008222
ACAGACG7350.030.66948570
TCGGCTC12700.030.61166820
CGAATGA450.00475576230.48930438
ATCAACG27150.030.465293
TCCTCGG12550.030.43112817
GACGAAT450.00483465330.38691936
GTAATAT13300.030.223271
GTATCCT12350.030.07009514
AACTGTC13100.030.06011230
GTATGCA450.0051352830.0143899
CTCGGCT12750.029.95755219
Produced by FastQC (version 0.11.5)