FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355662
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48540.35805385118119415No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC35610.26267609477878706No Hit
GCCTTGGGCTGACCCAGGACGGTCAGGTTGGTCCCTCCGCCGAAGAGCACATGGTTATCACCACTATCTCGGGAG34910.2575125658165531No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32600.24047292024118108No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26870.19820574745032316No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGGTCAACTTCATGGAAA24940.18396916045444958No Hit
AGCCAGGACAGGCCCCTGCAGTTGTTATTTATGGTAAAAACAACCGGCCC23860.17600257291271718No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGGTTGGTCCCTC22190.16368386810281618No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21960.16198728001522503No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21860.1612496330206202No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20190.14893092821071918No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19700.14531645793715542No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18300.13498940001268753No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17940.13233387083211007No Hit
ACTATAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGCAGACCAGTCCAAGAATGAGTTTTCCATGAAGTTGA17830.13152245913804472No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAA17660.1302684592472165No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17640.1301209298482955No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17310.12768669476609953No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17170.1266539889736527No Hit
CTCCTCACTCTTTGCATAGATTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA16100.11876116613138082No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15840.11684328394540823No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15830.11676951924594775No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15620.11522046055727755No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15600.1150729311583566No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15480.11418775476483077No Hit
GTCTATTACTGTGCGAGAGTAGAGTGGGTCCGCCTGGGGCTCTATTTCTA15460.11404022536590978No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14510.10703257891716371No Hit
GTCCTGGCTTCTGTTGGTACCAGCTTGCAAAATAGTTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT14400.10622116722309838No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14350.10585234372579597No Hit
GCCTATTATTGCAGCTCCCGAGATAGTGGTGATAACCATGTGCTCTTCGG14290.10540975552903306No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14210.10481963793334917No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14090.10393446153982334No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAACTATTTTGCAAGCTGGTA14090.10393446153982334No Hit
CTCTTTGCATAGATTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT14030.10349187334306044No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13990.1031968145452185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13700.10105763826086445No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGCCTATTATTGCAGCTCCCGA13660.1007625794630225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14700.050.608951
TATCGCG507.1859176E-848.09249536
AGTATCG1950.042.21484834
ACCGATC5000.041.6693427
TATACAC5850.040.249953
CCGATCA5350.040.23796528
GTATCGC603.025143E-740.05328435
GTATCAA43400.039.866341
ACGAGAC5450.039.44865422
TTATACA5900.039.3304632
ATCTCGT1750.039.27522737
CGATCAG5600.039.07201829
TCTCCGA5600.038.99419811
TCTCGTA1500.038.97833638
GTGGTAT3150.037.9180221
CCCACGA5700.037.7142819
ATCTCCG5800.037.6495710
CTTATAC6100.037.4828951
TCGTATG1400.036.94128440
TGCTGAT9200.036.3850270
Produced by FastQC (version 0.11.5)