FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355662
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50970.375978673150092No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45700.3371046765344164No Hit
GCCTTGGGCTGACCCAGGACGGTCAGGTTGGTCCCTCCGCCGAAGAGCACATGGTTATCACCACTATCTCGGGAG34040.2510950369634909No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC33760.2490296253785973No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30690.2263838626442284No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26930.1986483356470861No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23660.17452727892350747No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGGTCAACTTCATGGAAA22640.16700327957853803No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21310.1571925745502935No Hit
AGCCAGGACAGGCCCCTGCAGTTGTTATTTATGGTAAAAACAACCGGCCC21060.1553484570637814No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20780.1532830454788878No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGGTTGGTCCCTC20060.14797198711773288No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19850.1464229284290627No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18750.13830881148840934No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18220.13439928241700366No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17140.12643269487527128No Hit
CTCCTCACTCTTTGCATAGATTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA16820.12407222449253574No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAA16690.12311328339954944No Hit
ACTATAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGCAGACCAGTCCAAGAATGAGTTTTCCATGAAGTTGA16540.12200681290764218No Hit
GTCTATTACTGTGCGAGAGTAGAGTGGGTCCGCCTGGGGCTCTATTTCTA16320.1203839895195115No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15520.11448281356267269No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15410.11367140186860736No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15400.11359763716914688No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14970.11042575509234602No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14950.11027822569342506No Hit
GCCTATTATTGCAGCTCCCGAGATAGTGGTGATAACCATGTGCTCTTCGG14830.10939304929989925No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14470.10673752011932179No Hit
GTCCTGGCTTCTGTTGGTACCAGCTTGCAAAATAGTTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT14400.10622116722309838No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14400.10622116722309838No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAACTATTTTGCAAGCTGGTA14150.10437704973658625No Hit
CTCTTTGCATAGATTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT13830.10201657935385075No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGCCTATTATTGCAGCTCCCGA13590.1002462265667991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16000.048.591281
GTGGTAT3550.046.105881
TGCTGAT8500.044.48123670
CGTACGG350.001430709638.95319713
GTATCAA43150.037.8528141
TGGTATC4300.037.264282
CTAACGC400.002753114834.0903363
TATCAAC49800.032.3813672
ATCAACG51100.031.5552433
TCAACGC51400.031.237274
CAACGCA52750.030.5047075
CTGACGA450.00490023830.3036429
ATAAACG450.004901122330.3025253
CCGAAAC902.7650458E-630.18289268
ATCCGCA654.3513146E-429.8512168
AACGCAG53950.029.8261976
GGTCTGC7900.028.02704266
ACGCAGA57750.027.745547
CGCAGAG57950.027.5909548
AGAGTAC59250.027.1006611
Produced by FastQC (version 0.11.5)