FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004692285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524098
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG53400.3503711703578116No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG47990.3148747652709996No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA45390.29781549480413994No Hit
GTATATTACTGTGCGAAAAATGGCGACGCGATTCGGGGAGTTAGGTGGTC43520.28554594258374466No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATAACGAGAGTGTTTCTCTCTGTGTATGAG42070.27603211866953437No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36770.24125745194862797No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT35970.23600844565113266No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTCTGGGCTGAGCTGGCTTTTT34730.22787248589001496No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT33650.2207863273883963No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATAACGAGAGTGTTT32830.21540609593346358No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACATT30210.19821560030916646No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28780.18883300155239363No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28620.18778320029289455No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27240.17872866442971516No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATC25620.16809942667728714No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC25460.16704962541778812No Hit
GTGTATGAGCTGCAGTAATAATCAGCCTCGTCGTCACTCTGGAGACCAGA25450.1669840128390694No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC24670.1618662316990115No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24320.1595697914438573No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC23810.15622354992920404No Hit
CTCCTGCACTGGAACCAGCAATGACATTGGTGCTTATGACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC22970.15071209331683394No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22570.14808759016808629No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC21220.13922989204106298No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT21120.13857376625387605No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT20840.1367366140497527No Hit
CTCCTGTGCAGCCTCTGGATTCGTCTTTAGTAATTTTGCCATGAACTGGTTCCGCCAGACTCCAGGGAAGGGG20330.13339037253509944No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT20240.13279985932663124No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC19830.13010974359916488No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG19640.12886310460350975No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19350.1269603398206677No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAATGACATTGGTGCTTATGACTTTGTCTCCTGGTACCAACAACA19210.126041763718606No Hit
GATTATTACTGCAGCTCATACACAGAGAGAAACACTCTCGTTATTTTCGG19200.12597615113988733No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATAACGAGAGTGTTTC19140.1255824756675752No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT18860.12374532346345184No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18270.11987418131904903No Hit
CTCCAGGGAAGGGGCTGGAGTGGGTGTCAGTTATTAATGGAAGTGGAAGT18070.1185619297446752No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC17850.117118453012864No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG17830.11698722785542662No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17650.11580620143849017No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17290.11344414860461728No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC17250.11318169828974252No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTCTGGGCTGAGCTGGC17180.11272241023871168No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17160.1125911850812743No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCAGGGGTCGGACCACC17140.11245995992383692No Hit
GAACAGAGAGAACTCACCATGGAGTCTGGGCTGAGCTGGCTTTTTCTTGT17100.11219750960896216No Hit
GGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16840.11049158256227617No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT16590.10885126809430891No Hit
GCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAG16530.10845759262199674No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC16450.10793269199224723No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16360.107342178783779No Hit
CTCCAGCCCCTTCCCTGGAGTCTGGCGGAACCAGTTCATGGCAAAATTAC16110.10570186431581172No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15940.10458645047759396No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA15720.10314297374578275No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG15640.10261807311603321No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15280.10025602028216032No Hit
GGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACCAGGGA15280.10025602028216032No Hit
GTAATAATCAGCCTCGTCGTCACTCTGGAGACCAGAGATGGTCAGGGAGG15250.10005918254600427No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)