FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524098
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG56260.36913636787135734No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA47190.3096257589735043No Hit
GTATATTACTGTGCGAAAAATGGCGACGCGATTCGGGGAGTTAGGTGGTC41880.27478547967387923No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG40820.26783054632969794No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATAACGAGAGTGTTTCTCTCTGTGTATGAG39780.26100683814295406No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35490.2328590418726355No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT32250.2116005663677795No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTCTGGGCTGAGCTGGCTTTTT31310.20543298396822254No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30130.19769069967941694No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACATT28200.18502747198670952No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAATC27900.18305909462514877No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC27550.18076265436999459No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATAACGAGAGTGTTT26860.1762353864384049No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT26680.17505436002146843No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26430.17341404555350115No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25810.1693460656729423No Hit
GTGTATGAGCTGCAGTAATAATCAGCCTCGTCGTCACTCTGGAGACCAGA24790.16265358264363577No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23220.15235240778480125No Hit
CTCCTGCACTGGAACCAGCAATGACATTGGTGCTTATGACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC23160.1519587323124891No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC22960.15064648073811526No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22060.14474134865343305No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC21790.1429698090280284No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC21400.1404109184579994No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21100.13844254109643866No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT19900.13056903165019573No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19820.1300441310204462No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT19480.1278133033440107No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAATGACATTGGTGCTTATGACTTTGTCTCCTGGTACCAACAACA19460.12768207818657332No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC19230.1261729888760434No Hit
CTCCTGTGCAGCCTCTGGATTCGTCTTTAGTAATTTTGCCATGAACTGGTTCCGCCAGACTCCAGGGAAGGGG19150.1256480882462939No Hit
GATTATTACTGCAGCTCATACACAGAGAGAAACACTCTCGTTATTTTCGG19140.1255824756675752No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT18980.12453267440807611No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG18820.12348287314857707No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC18760.1230891976762649No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18390.12066153226367334No Hit
GGACACAGGGTCCTGGGCCCAATCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18230.11961173100417427No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18070.1185619297446752No Hit
CTCCAGCCCCTTCCCTGGAGTCTGGCGGAACCAGTTCATGGCAAAATTAC17770.11659355238311447No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17630.11567497628105279No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17500.11482201275770981No Hit
GTAATATACGGCCGTGTCTTCGACTCTCAGGCTGTCCATTTGCAGATACAGCGTACTCTTGGAATTGTCTCTGGA17200.11285363539614907No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG16950.11121332092818177No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC16890.11081964545586963No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT16730.10976984419637056No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA16730.10976984419637056No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16720.10970423161765189No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16550.10858881777943412No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG16480.1081295297284033No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC16330.10714534104762291No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCAGGGGTCGGACCACC16280.10681727815402946No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16200.10629237752427993No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15420.10117459638422201No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT15340.1006496957544725No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT15270.10019040770344165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT150.002465752468.209956
AACGTAC352.12259E-549.8603950
GGATGGG18050.048.13811570
AGCGTAA605.0658855E-946.53636650
GGTATCA10450.041.1393471
GTGGTAT3750.040.0335921
TGGTATC3700.038.709952
AATGGTA4050.038.6878170
CGTAATC654.8651236E-737.69849452
GTATCAA26950.037.094711
AAGCGTA951.9645086E-1036.70348449
GTTCGAC853.3051037E-936.13368618
TAAAGCG1550.035.8979347
ACCCTGG15700.034.92991670
AGGATGG17000.033.51020469
TATCAAC30150.033.027112
AGCGGAC1400.032.40925250
ATCAACG30900.032.2233663
TCAACGC30900.032.1140674
CGTGTGT3000.031.82191313
Produced by FastQC (version 0.11.5)