FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335252
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48510.3633022081225117No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47560.3561874462648249No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38080.28518961214811883No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37070.2776254969099466No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33210.24871709609871395No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31280.2342628956930976No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28330.21216968781922813No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28070.21022248983712438No Hit
GATCTATGCTGCTTCCAATTTACAAAGTGGTGTCCCATCAAGGTTTAGTGGCAGTGGATCTGGGACAGAATTCA27480.20580384826235049No Hit
CACCATCACCGGTCTGCAACCTGCAGATTTTGCAACTTACTTCTGTCAACATGGTTTCGCTACCCCATTCACTTT26690.19988736208595828No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26670.19973757762579647No Hit
GAATTCACTCTCACCATCACCGGTCTGCAACCTGCAGATTTTGCAACTTACTTCTGTCAACATGGTTTCGCTACC26380.19756570295345No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26380.19756570295345No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25730.19269770799819058No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24980.18708079074212208No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23900.17899242989338343No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAATATTTACAACTTCTTAAATTGGTATCAGGTGAAA23280.17434911162836678No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23070.1727763747966676No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22650.16963090113326923No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAATATTTACAA20780.1556260541081384No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20690.15495202403741018No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19690.1474628010293188No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19520.14618963311794328No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19240.1440926506756777No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19210.14386797398543497No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCACCTGATACCAATT18960.14199566823341211No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18960.14199566823341211No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18550.13892508680009466No Hit
GTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCACTAAACCTTGA18490.13847573341960917No Hit
GTGCAGCCACAGTTCGTCTGATATCCACTGTGGTCCCAGGGCCGAAAGTG18360.1375021344285573No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATATCCACTGTGGTCC17810.13338306177410705No Hit
GTGGTGTCCCATCAAGGTTTAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCGGTCTGCAACCTG17730.13278392393345975No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17530.13128607933184147No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17230.12903931242941408No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17110.12814060566844312No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16970.1270921144473103No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16880.1264180843765821No Hit
GATATCCACTGTGGTCCCAGGGCCGAAAGTGAATGGGGTAGCGAAACCATGTTGACAGAAGTAAGTTGCAAAAT16860.12626829991642027No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16660.124770455314802No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16360.1225236884123746No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16090.12050159820018994No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTAAACCTTGATGGGACACCA16020.11997735258962353No Hit
GTGGATATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC15700.11758080122703429No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15200.11383618972298862No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15190.1137612974929077No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14950.11196388397096578No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14910.11166431505064212No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14900.11158942282056121No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14820.11099028497991391No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14680.1099417937587811No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14550.10896819476772925No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14310.10717078124578731No Hit
GGTGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGCTGCTTCCAATT14280.10694610455554458No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14210.10642185894497817No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14140.10589761333441178No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCACCTGATACCAATTTAAGA14000.104849122113279No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13410.10043048053850509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAA350.004784565457.69701870
GTCGAAC200.00647020953.46253658
TACGTAT650.001057031341.42349670
TCGTATG2100.041.0730640
CGAGTTC801.1350494E-940.3046560
GTATCAA43300.039.570651
CTAACGC451.1060675E-437.693123
TTGCCGA852.246452E-937.58740256
CGTATGC2150.036.983141
GTGGTAT4300.035.5069771
TATCTCG2150.035.095236
ATGCCGT2200.034.72089844
ATCAACG51200.033.2612343
TCAACGC51350.033.162834
TATCAAC51450.033.1008532
GACGGAC2800.032.76500726
CCGTCTT2150.032.46025547
CAACGCA52700.032.3776745
CGGACTC2950.032.30533628
AACGCAG53000.032.194416
Produced by FastQC (version 0.11.5)