FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335252
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48590.36390134596315904No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41740.3126001683577332No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39500.2958243088196086No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36650.2744800232465482No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29950.22430222909233613No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29400.22018315643788589No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28950.21681300608424478No Hit
GATCTATGCTGCTTCCAATTTACAAAGTGGTGTCCCATCAAGGTTTAGTGGCAGTGGATCTGGGACAGAATTCA27700.20745147732413055No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26760.2004116076965247No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25910.19404576813964705No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25850.19359641475916156No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25680.19232324684778604No Hit
CACCATCACCGGTCTGCAACCTGCAGATTTTGCAACTTACTTCTGTCAACATGGTTTCGCTACCCCATTCACTTT25470.19075051001608684No Hit
GAATTCACTCTCACCATCACCGGTCTGCAACCTGCAGATTTTGCAACTTACTTCTGTCAACATGGTTTCGCTACC25220.18887820426406401No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25050.1876050363526885No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24510.18356085592831914No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23330.17472357277877135No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23300.17449889608852862No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23150.1733755126373149No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAATATTTACAACTTCTTAAATTGGTATCAGGTGAAA21890.16393909164711978No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21530.1612429713642069No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAATATTTACAA20480.15337928720571098No Hit
GTGGTGTCCCATCAAGGTTTAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCGGTCTGCAACCTG20010.14985935239190806No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19300.1445420040561632No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18970.14207056046349303No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18910.14162120708300754No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18480.13840084118952828No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17870.13383241515459254No Hit
GTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCACTAAACCTTGA17800.13330816954402613No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCACCTGATACCAATT17530.13128607933184147No Hit
GTGCAGCCACAGTTCGTCTGATATCCACTGTGGTCCCAGGGCCGAAAGTG17500.13106140264159874No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17450.13068694149119417No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC17410.13038737257087052No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTAAACCTTGATGGGACACCA17100.1280657134383622No Hit
GTGGATATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC16640.12462067085464018No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16560.12402153301399287No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16500.12357217963350738No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16230.12155008942132271No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16210.12140030496116089No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16140.1208760593505945No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATATCCACTGTGGTCC15820.11847950798800526No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15780.11817993906768161No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15590.11675698669614426No Hit
GATATCCACTGTGGTCCCAGGGCCGAAAGTGAATGGGGTAGCGAAACCATGTTGACAGAAGTAAGTTGCAAAAT15360.11503446540428325No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15010.11241323735145126No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14860.11128985390023757No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14800.11084050051975208No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14470.10836905692708193No Hit
GGTGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGCTGCTTCCAATT14250.10672142786530184No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14110.10567293664416903No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14020.10499890657344083No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13840.10365084643198437No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13840.10365084643198437No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCACCTGATACCAATTTAAGA13780.1032014930514989No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13660.10230278629052793No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13450.10073004945882874No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13410.10043048053850509No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13370.10013091161818143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG705.329639E-1043.5352862
CTAACGC751.0495569E-940.6253243
GTGGTAT4700.038.20061
GTATCAA46500.038.028541
CCGTCAT800.002439393234.9391370
TTAACGG1700.034.23780435
CGGTACC1650.033.73694245
TAACGGC1750.033.301236
GCGGTAC1700.032.6868144
TCAACGC54100.032.2912064
ATCAACG54400.032.0496943
TATCAAC54750.032.0363122
CAACGCA55550.031.512815
AACGCAG55850.031.4647926
AACGGCC1950.029.91859437
CGCAGAG58500.029.8079458
AGAGTAC59200.029.2256111
AGTACTA500.006286951228.79716561
CGCGGTA2050.028.7604943
GCAGAGT60650.028.751279
Produced by FastQC (version 0.11.5)